Genotyping of gas- and aroma- forming starter cultures using molecular methods

The article presents the results of studies on the resolving power of the RAPD and Rep-PCR methods for revealing the genetic heterogeneity of gas- and aroma-forming lactic acid bacteria isolated from raw milk. The study of the typing ability of Rep-PCR in relation to leuconostocs showed that isolates obtained from different natural sources form different amplicon profiles when using primers BOXA1R, BOXA2R, ERIC2-1. The typing ability of Rep-PCR for diacetyl lactococci isolates was demonstrated only with primers BOXA2R and ERIC2-1. The RAPD-PCR method showed typing ability for leuconostocs using primers XD8, P15, P16, and RAPD-mes. The analysis of the fragment profiles of diacetyl lactococcal isolates obtained by RAPD-PCR with primers XD8 and P16 confirmed the ability of the method to detect heterogeneity within the biovariant of Lactococcus lactis subsp. lactis biovar. diacetylactis. The results of the research are used to isolate new strains and replenish the Republican collection of industrial strains of starter cultures and their bacteriophages of RUE “Institute of Meat and Dairy Industry”.

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