Optimization of in vitro micropropagation stage of pear varieties regionalized in Belarus
https://doi.org/10.29235/1817-7204-2025-63-3-204-218
Abstract
The objects of the study were 9 pear varieties regionalized in the Republic of Belarus including 8 varieties of Belarusian selection (Belorusskaya pozdnyaya, Viliya, Zaveya, Kupala, Prosto Maria, Spakusa, Yasachka (P. communis × P.× ussuriensis), Kudesnitsa (P. communis × P.× pyrifolia)) and 1 introduced variety Talgarskaya krasavitsa (P. communis × P.× pyrifolia). Optimal nutrient media for pear propagation were determined: agarized DKW medium for varieties Kupala, Prosto Maria and Yasachka, and DKW and modified MS medium (the NH4NO3 content was reduced to ¼) for varieties Spakusa, Talgarskaya krasavitsa, Belorusskaya pozdnyaya, and Kudesnitsa. The optimal concentration of 6-BA for propagation of pear varieties made 1.0 mg/l. Increasing the concentration of 6-BA to 2.0 mg/l allows increasing the number of shoots per explant, but reduces the height of the shoots (i. e. readiness for rooting) and results in narrow and unexpanded leaves with symptoms of vitrification. Stimulation of growth of axillary buds and increase in propagation coefficient of all studied pear varieties, maintaining the quality of the shoots, can be achieved by removing the apical bud and leaves of microshoots and placing them horizontally (shoot length 1.5–2 cm) on nutrient medium. The use of large-volume culture vessels (250–450 ml) has a positive effect on proliferation rate of pear varieties.
About the Authors
E. V. Kolbanova
Institute for Fruit Growing, National Academy of Sciences of Belarus
Belarus
Belarus
Elena V. Kolbanova – Ph. D. (Biology), Associate Professor, Head of Diagnostics Laboratory of Biotechnology Department
2, Kovalyov St., 223013, Samokhvalovichy, Minsk District, Minsk Region
N. V. Kukharchyk
Institute for Fruit Growing, National Academy of Sciences of Belarus
Belarus
Belarus
Natallia V. Kukharchyk – Dr. Sc. (Agriculture), Professor, Head of Biotechnology Department
2, Kovalyov St., 223013, Samokhvalovichy, Minsk District, Minsk Region
T. N. Bazhydai
Institute for Fruit Growing, National Academy of Sciences of Belarus
Belarus
Belarus
Tatsiana N. Bazhydai – Ph. D. (Biology), Associate Professor, Leading Researcher of Biotechnology Department
2, Kovalyov St., 223013, Samokhvalovichy, Minsk District, Minsk Region
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