Polymorphism of wild species of р. Malus MILL. according to powdery mildew resistance genes

Powdery mildew (Podosphaera leucotricha (Ell. E tEv.) Salm.) – is one of the most widespread apple diseases in world. Identification of forms, carrying resistance genes, is an important stage in breeding programs aimed at obtaining powdery mildew resistant apple varieties. Diagnostic DNA markers of target genes will increase reliability of identification and efficiency of apple breeding for the creation of resistant genotypes. The purpose of this study was molecular genetic testing of wild species of genus Malus Mill. According to Pl-1, Pl-w and Pl-d powdery mildew resistance genes for revealing polymorphism of the studied loci and identification of valuable genotypes for breeding. The study subjects were the wild species of genus Malus Mill. of different ecological and geographic origin. The Pl-1 gene was identified using AT20-SCAR marker, Pl-w gene - EM M02 marker, Pl-d gene - EM DM01 marker. AT20-SCAR marker (Pl-1 gene) was identified in 37.3 % of genotypes. EM M02 marker (Pl-w gene) was detected in 16.4 % of the studied forms. EM DM01 marker (Pl-d gene) is present in 10.4 % of the analyzed forms. At least one of the studied molecular markers is present in the genome of 52.2 % of apple wild species. The Pl-1 gene in apple wild species is most spread in Baccatae series, the Pl-w gene - in Baccatae and Sieboldinae series, the Pl-d gene - in Orientalis series. Wild species M. baccata 2319, M. mandshurica 41947, M. sachalinensis 85, M. sachalinensis 97, M. purpurea v. pendula 2396 are characterized by combination of Pl-1 and Pl-w genes; M. turkmenorum 13283 and M. turkmenorum 29 421 - Pl-1 and Pl-d genes; M. denticulata 29416 - Pl-w and Pl-d genes, which allows to recommend them as promising complex sources of high powdery mildew resistance for breeding.

1. Bus V. G. M., Ranatunga C., Alspach P. A., Oraguzie N. C., Whitworth C. A partial diallel study of powdery mildew resistance in six apple cultivars under three growing conditions with different disease pressures. Euphytica, 2006, vol. 148, no. 3, pp. 235-242. https://doi.org/10.1007/s10681-005-9014-2

2. Pessina S., Angeli D., Martens S., Visser R. G., Bai Y., Salamini F., Velasco R., Schouten H. J., Malnoy M. A. The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica). Plant Biotechnology Journal, 2016, vol. 14, no. 10, pp. 2033-2044. https://doi.org/10.1111/pbi.12562

3. Radwan M., Darwesh D. Effect of integrated control program of powdery mildew disease on growth and productivity of apple. Journal of Plant Protection and Pathology, 2018, vol. 9, no. 12, pp. 787-794. https://doi.org/10.21608/jppp.2018.44066

4. Susuri L. R., Susuri H. S., Muja I. Z. Podosphaera leucotricha and reaction of some apple cultivars. Agronomski Glasnik, 2000, vol. 63, no. 1/2, pp. 29-39.

5. Urbanovich O. Yu., Kozlovskaya Z. A., Kartel’ N. A. Spread of mildew resistance genes in the collection of apple cultivars and species grown in Belarus. Molekulyarnaya i prikladnaya genetika: sbornik nauchnykh trudov [Molecular and applied genetics: a collection of scientific papers]. Minsk, 2010, vol. 11, pp. 20-25 (in Russian).

6. Ul’yanovskaya E. V., Suprun I. I., Sedov E. N., Sedysheva G. A., Serova Z. M. Creation of the apple-tree genotypes immune to scab and with complex of valuable agrobiological traits. Plodovodstvo i vinogradarstvo Yuga Rossii = Fruit Growing and Viticulture of South of Russia, 2011, no. 10 (4), pp. 14-30 (in Russian).

7. Lespinasse Y., Durel C. E., Laurens F., Chevalier M., Pinet C., Parisi L. A European project: D. A. R.E - durable apple resistance in Europe (FAIR5 CT97-3898) durable resistance of apple to scab and powdery-mildew: one step more towards an environmental friendly orchard. Acta Horticulturae, 2000, no. 538, pp. 197-200. https://doi.org/10.17660/ActaHortic.2000.538.32

8. Yakuba G. V. Apple technologies protection from diseases with domestic fungicides application. Sadovodstvo i vinogradarstvo = Horticulture and Viticulture, 2016, no. 4, pp. 33-39 (in Russian). https://doi.org/10.18454/VSTISP.2016.4.2841

9. Rather T. R., Bhat Z. A., Pandit B. A., Shiekh K., Malik A. R., Ganai M. A. Bioefficacy studies of new fungicide molecules (Proquinazid 20 EC) against powdery mildew of apple. Journal of Pharmacognosy and Phytochemistry, 2019, vol. 8, no. 1, pp. 1963-1965.

10. Suprun I. I., Nasonov A. I., Yakuba G. V., Lobodina E. V., Barsukova O. N. Effective selection of apple seedlings in a seed plot on resistance to scab and powder mildew. Plodovodstvo i vinogradarstvo Yuga Rossii = Fruit Growing and Viticulture of South of Russia, 2016, no. 38 (2), pp. 117-129 (in Russian).

11. Nasir M., Idress M., Iqbal B., Hussain M., Mohy-ud-Din G., Ayub M. Comparative efficacy of bio control agent bacillus subtilis and fungicides against powdery mildew of apple. Journal of Agricultural Research, 2017, vol. 55, no. 1, pp. 75-84.

12. Palmer C. «Greening» agriculture in the developing world. Rural 21, 2008, vol. 42, no. 3, pp. 30-32.

13. Zhuchenko A. A. Biologization and greening of intensification processes in agriculture. Vestnik OrelGAU, 2009, no. 3 (18), pp. 8-12 (in Russian).

14. Gorgitano M. T., Pirilli M. Life cycle economic and environmental assessment for a greening agriculture. Quality - Access to Success, 2016, vol. 17, suppl. 1, pp. 181-185.

15. Kazlouskaya Z., Hashenka T., Vaseha V., Yarmolich S. Breeding of new apple cultivars in Belarus. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences, 2013, vol. 67, no. 2, pp. 94-100. https://doi.org/10.2478/prolas-2013-0015

16. Savel’ev N. I., Lyzhin A. S., Savel’eva N. N. Genetic diversity of genus Malus Mill. for scab resistance genes. Russian Agricultural Sciences, 2016, vol. 42, no. 5, pp. 310-313. https://doi.org/10.3103/S1068367416050189

17. Dan C., Sestras A., Bozdog C., Sestras R. Investigation of wild species potential to increase genetic diversity useful for apple breeding. Genetika, 2015, vol. 47, no. 3, pp. 993-1011. https://doi.org/10.2298/gensr1503993d

18. Savel’ev N. I., Lyzhin A. S., Savel’eva N. N. Selection of promising apple genotypes for columnar growth habit and scab resistance using diagnostic DNA markers. Vavilovskii zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding, 2016, vol. 20, no. 3, pp. 329-332 (in Russian). https://doi.org/10.18699/VJ16.122

19. Lyzhin A. S. Creation of genetic passports of apple rootstock forms on the basis of microsatellite DNA polymorphism. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology in Agro-Industrial Complex, 2019, vol. 33, no. 2, pp. 11-13 (in Russian). https://doi.org/10.24411/0235-2451-2019-10203

20. Dunemann F., Peil A., Urbanietz A., Garcia-Libreros T. Mapping of the apple powdery mildew resistance gene Pl1 and its genetic association with an NBS-LRR candidate resistance gene. Plant Breeding, 2007, vol. 126, no. 5, pp. 476-481. https://doi.org/10.1111/j.1439-0523.2007.01415.x

21. Evans K., James C. Identification of SCAR markers linked to Pl-w mildew resistance in apple. Theoretical and Applied Genetics, 2003, vol. 106, no. 7, pp. 1178-1183. https://doi.org/10.1007/s00122-002-1147-2

22. James C. M., Clarke J. B., Evans K. M. Identification of molecular markers linked to the mildew resistance gene Pl-d in apple. Theoretical and Applied Genetics, 2004, vol. 110, no. 1, pp. 175-181. https://doi.org/10.1007/s00122-004-1836-0

23. Markussen T., Kruger J., Schmidt H., Dunemann F. Identification of PCR-based markers linked to the powdery-mildew-resistance gene Pl1 from Malus robusta in cultivated apple. Plant Breeding, 1995, vol. 114, no. 6, pp. 530-534. https://doi.org/10.1111/j.1439-0523.1995.tb00850.x

24. Patzak J., Paprštein F., Henychová A. Identification of apple scab and powdery mildew resistance genes in Czech Apple (Malusçdomestica) genetic resources by PCR molecular markers. Czech Journal of Genetics and Plant Breeding, 2011, vol. 47, no. 4, pp. 156-165.