Simultaneous direct ex vitro rooting and adaptation of the blue honeysuckle micro-sprouts (Lonicera Caerulea L. var. Kamtschatica)

Direct rooting of regenerative plants under ex vitro conditions is of great importance in agricultural biotechnology, as it leads to acceleration of micropropagation process by eliminating the stage of in vitro rooting and reduces the costs for obtaining healthy planting material of fruit and small-fruit crops. Possibility of direct ex vitro rooting and adaptation of blue honeysuckle microshoots at one stage with no stage of in vitro rooting has been determined. For ex vitro rooting and adaptation of honeysuckle microshoots, it is required to use non-sterile substrate: Sphagnum L. moss with a surface layer of peat (0.5 cm). The rate of rooted microshoots on this substrate makes 72-84 % during summer-autumn period and not less than 60 % during winter period. Thermal treatment of the peat surface layer and the use of aqueous IBA solutions at ex vitro rooting stage will be economically justified during propagation of individual varieties characterized by low proliferation activity in in vitro culture or low ex vitro rhizogenic activity, as well as breeding novelties requiring rapid propagation and obtaining of large amount of planting material. Thermal treatment of the peat surface layer allows increasing the rate of rooted microshoots of Volkhov variety by 10.7 %, Krupnoplodnaya variety by 13.2 %, Pavlovskaya variety by 3.8 % when rooting during spring period. Use of aqueous solutions of IBA increases the rate of rooted plants by 6.2-6.7 % in Vostorg and Krupnoplodnaya varieties when rooting during summer period. Carrying out of one cutting of ex vitro rooted microshoots and further cuttings of obtained material can be used to increase the yield of planting material of blue honeysuckle varieties. Ex vitro rooting and adaptation of blue honeysuckle microshoots at one stage with further ex vitro cutting increases production profitability by 3.5 and 12.9 times in comparison with conventional method of clonal micropropagation of blue honeysuckle.

1. Sedlák J., Paprštein F. In vitro propagation of blue honeysuckle. Horticultural Science, 2007, vol. 34, no. 4, pp. 129- 131. https://doi.org/10.17221/1871-hortsci

2. Sorokopudov V. N., Kuklina A. G., Upadyshev M. T. Edible honeysuckle varieties: biology and cultivation basics. Moscow, All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery, 2018. 159 p. (in Russian).

3. Marcelina K-M., Ireneusz O. Propagation of Blue Honeysuckles (Lonicera caerulea L.) in in vitro culture. Journal of Basic & Applied Sciences, 2014, vol. 10, pp. 164-169. https://doi.org/10.6000/1927-5129.2014.10.22

4. Makarov S. S., Kalashnikova E. A. Influence of nutrient medium composition on clonal micropropagation of honeysuckle. Plodovodstvo i yagodovodstvo Rossii: sbornik nauchnykh rabot = Pomiculture and small fruits culture in Russia: a collection of scientific works. Moscow, 2017, vol. 49, pp. 217-222 (in Russian).

5. Dziedzic E. Propagation of blue honeysuckle (Lonicera caerulea var. kamtschatica Pojark.) in in vitro culture. Journal of Fruit and Ornamental Plant Research, 2008, vol. 16, pp. 93-100.

6. Ninjmaa O., Gereltuya P., Saranchimeg B., Narangoo A. In vitro propagation of blue honeysuckle (Lonicera edulis). International Journal of Research Studies in Science, Engineering and Technology, 2015, vol. 2, iss. 10, pp. 57-61.

7. Pan’kova O. A. Perspectives of use of biotechnological methods in system of production of health-improved planting stock of honeysuckle in Udmurtija. Agrarnaya nauka Evro-Severo-Vostoka = Agricultural Science Euro-North-East, 2009, no. 1 (12), pp. 43-47 (in Russian).

8. Vysotskii V. A., Valikov V. A. Clonal micropropagation of honey suckle for commercial purposes. Sadovodstvo i vinogradarstvo = Horticulture and Viticulture, 2014, no. 6, pp. 18-23 (in Russian).

9. Hui J. X., Wen S. C., Hua Z. Y., Ming L. X. Comparative study on different methods Lonicera japonica Thunb. micropropagation and acclimatization. Journal of Medicinal Plants Research, 2012, vol. 6, no. 27, pp. 4389-4393. https://doi.org/10.5897/jmpr011.1715

10. Palacios N., Christou P., Leech M. J. Regeneration of Lonicera tatarica plants via adventitious organogenesis from cultured stem explants. Plant Cell Reports, 2002, vol. 20, no. 9, pp. 808-813. https://doi.org/10.1007/s00299-001-0415-y

11. Kolbanova E. V. Various substrates and season of the year effect on honeysuckle (Lonicera caerulea L. var. kamtschatica) plant regenerants ex vitro adaptation. Plodovodstvo: sbornik nauchnykh trudov = Fruit-growing: a collection of scientific works. Minsk, 2018, vol. 30, pp. 159-164 (in Russian).

12. Isutsa D. K., Pritts M. P., Mudge K. W. Rapid propagation of blueberry plants using ex vitro rooting and controlled acclimatization of micropropagules. HortScience, 1994, vol. 29, no. 10, pp. 1124-1126. https://doi.org/10.21273/hortsci.29.10.1124

13. Meiners J., Schwab M., Szankowsk I. Efficient in vitro regeneration for Vaccinium species. Plant Cell, Tissue and Organ Culture, 2007, vol. 89, no. 2-3, pp. 169-176. https://doi.org/10.1007/s11240-007-9230-7

14. Liu C., Callow P., Hancock J. F., Song G., Rowland L. J. Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars. Plant Cell, Tissue and Organ Culture, 2010, vol. 103, no. 1, pp. 137-144. https://doi.org/10.1007/s11240-010-9755-z

15. Mihaljević S., Salopek-Sondi B. Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries. Plant, Soil and Environment, 2012, vol. 58, no. 5, pp. 236-241. https://doi.org/10.17221/34/2012-PSE

16. Bozhidai T. N., Kukharchik N. V. Peculiarities of in vitro propagation and ex vitro rooting of blueberry cv. Northblue. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya biyalagichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Biological series, 2014, no. 4, pp. 28-31 (in Russian).

17. Bozhidai T. N. Ex vitro rooting of the in vitro-derived cranberry plants. Subtropicheskoe i dekorativnoe sadovodstvo = Subtropical and ornamental horticulture, 2018, iss. 65, pp. 105-109 (in Russian). https://doi.org/10.31360/2225-3068-2018-65-105-109

18. Dednath S. C., McRae K. B. In vitro culture of lingonberry (Vaccinium vitis-idaea L.): the influence of cytokinins and media types on propagation. Small Fruits Review, 2001, vol. 1, no. 3, pp. 3-19. https://doi.org/10.1300/J301v01n03_02

19. Marcotrigianol M., McGlew S. P. A two-stage micropropagation system for cranberries. Journal of the American Society for Horticultural Science, 1991, vol. 116, no. 5, pp. 911-916.

20. Clara D., Fira A., Joshee N. An efficient ex vitro rooting and acclimatization method for horticultural plant using float hydroculture. HortScience, 2013, vol. 48, no. 9, pp. 1159-1167. https://doi.org/10.21273/hortsci.48.9.1159

21. Fira A., Clara D., Cristea V., Plopa C. In vitro propagation of Lonicera kamtschatica. Agricultura - Ştiinţă şi Practică = Agriculture - Science and Practice, 2014, vol. 23, no. 1-2 (89-90), pp. 90-99. https://doi.org/10.15835/arspa.v89i1-2.10239

22. Yarmolenko L. V., Matushkina O. V., Pronina I. N. Peculiarities of raspberry varieties rhizogenesis ex vitro. Plodovodstvo i yagodovodstvo Rossii: sbornik nauchnykh rabot = Pomiculture and small fruits culture in Russia: a collection of scientific works. Moscow, 2017, vol. 48, pt. 1, pp. 308-311 (in Russian).

23. Zaitseva Yu. G., Ambros E. V., Novikova T. I. Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron. Turczaninowia, 2018, vol. 21, no. 1, pp. 144-152. https://doi.org/10.14258/turczaninowia.21.1.13

24. Vasar V. Effect of ascorbic acid and citric acid on ex vitro rooting and acclimatization of Prunus avium L. microshoots. Acta Horticulturae, 2003, no. 616, pp. 251-254. https://doi.org/10.17660/ActaHortic.2003.616.33

25. Borkowska, B. Morphological and physiological characteristics of micropropagated strawberry plants rooted in vitro or ex vitro. Scientia Horticulturae, 2001, vol. 89, no. 3, pp. 195-206. https://doi.org/10.1016/S0304-4238(00)00230-2

26. Lepse L., Laugale V. Micropropagation, rooting and acclimatization of blackberry ‘Agavam’. Acta Horticulturae, 2009, no. 839, pp. 43-49. https://doi.org/10.17660/ActaHortic.2009.839.2

27. Fira A., Clara D., Plopa C. New aspects regarding the micropropagation of blackberry cultivar ‘Thornless evergreen’. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 2010, vol. 67, no. 1, pp. 106-114.

28. Fira A., Clara D., Rakosy-Tican E. In vitro propagation of the thornless blackberry cultivar ‘Loch Ness’. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 2011, vol. 68, no. 1, pp. 39-46.

29. Fira A., Clara D., Vescan L. A. Direct ex vitro rooting and acclimation in blackberry cultivar ‘Loch Ness’. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 2012, vol. 69, no. 1-2, pp. 247-254.

30. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 1962, vol. 15, no. 3, pp. 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x