vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2020-58-3-298-310vestiag-506Research ArticleЗЕМЛЯРОБСТВА І РАСЛІНАВОДСТВAAGRICULTURE AND PLANT CULTIVATIONОдновременное прямое укоренение ex vitro и адаптация микропобегов жимолости синей (Lonicera caerulea L. var. kamtschatica)Simultaneous direct ex vitro rooting and adaptation of the blue honeysuckle micro-sprouts (Lonicera Caerulea L. var. Kamtschatica)КолбановаЕ. В.KolbanovaE. V.

Колбанова Елена Вячеславовна – кандидат биологических наук, доцент, заведующая лабораторией диагностики отдела биотехнологии

ул. Ковалева, 2, 223013 аг. Самохваловичи, Минский район

Elena V. Kolbanova - Ph.D. (Biol.), Associate Professor

2, Kovalev Str., 223013 Samokhvalovichy, Minsk Region

kolbanova@tut.byИнститут плодоводства, Национальная академия наук БеларусиInstitute for Fruit Growing, the National Academy of Sciences of Belarus202004082020583298310Copyright © Колбанова Е.В., 20202020Колбанова Е.В.Kolbanova E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/506

Прямое укоренение растений-регенерантов в условиях ex vitro имеет большое значение в сельскохозяйственной биотехнологии, так как ведет к ускорению процесса микроразмножения за счет исключения этапа укоренения in vitro и сокращению затрат на получение оздоровленного посадочного материала плодовых и ягодных культур. Установлена возможность применения прямого укоренения ex vitro и адаптации микропобегов жимолости синей в одной стадии без стадии укоренения in vitro. Для укоренения ex vitro и адаптации микропобегов жимолости необходимо использовать нестерильный субстрат – мох Sphagnum L. с поверхностным слоем (0,5 см) торфа. Выход укорененных микропобегов на этом субстрате составляет 72–84 % в летне-осенний период и не менее 60 % в зимний период. Термическая обработка поверхностного слоя торфа и использование водных растворов ИМК на стадии укоренения ex vitro экономически будут оправданы при размножении отдельных сортов, характеризующихся низкой пролиферационной активностью в культуре in vitro или низкой ризогенной активностью ex vitro, а также селекционных новинок, требующих быстрого размножения и получения большого количества посадочного материал. Термическая обработка поверхностного слоя торфа позволяет увеличить выход укоренившихся микропобегов сорта Волхова на 10,7 %, сорта Крупноплодная – на 13,2 %, сорта Павловская – на 3,8 % при укоренении в весенний период. Применение водных растворов ИМК увеличивает выход укорененных растений на 6,2–6,7 % у сортов Восторг и Крупноплодная при укоренении в летний период. Проведение одного черенкования укоренившихся ex vitro микропобегов и в последующем черенкование их черенков можно использовать для увеличения выхода товарной продукции сортов жимолости синей. Одновременное прямое укоренение ex vitro и адаптация микропобегов жимолости синей с последующим черенкованием ex vitro повышает рентабельность производства в 3,5 и 12,9 раза по сравнению с классическим способом клонального микроразмножения жимолости синей.

Direct rooting of regenerative plants under ex vitro conditions is of great importance in agricultural biotechnology, as it leads to acceleration of micropropagation process by eliminating the stage of in vitro rooting and reduces the costs for obtaining healthy planting material of fruit and small-fruit crops. Possibility of direct ex vitro rooting and adaptation of blue honeysuckle microshoots at one stage with no stage of in vitro rooting has been determined. For ex vitro rooting and adaptation of honeysuckle microshoots, it is required to use non-sterile substrate: Sphagnum L. moss with a surface layer of peat (0.5 cm). The rate of rooted microshoots on this substrate makes 72-84 % during summer-autumn period and not less than 60 % during winter period. Thermal treatment of the peat surface layer and the use of aqueous IBA solutions at ex vitro rooting stage will be economically justified during propagation of individual varieties characterized by low proliferation activity in in vitro culture or low ex vitro rhizogenic activity, as well as breeding novelties requiring rapid propagation and obtaining of large amount of planting material. Thermal treatment of the peat surface layer allows increasing the rate of rooted microshoots of Volkhov variety by 10.7 %, Krupnoplodnaya variety by 13.2 %, Pavlovskaya variety by 3.8 % when rooting during spring period. Use of aqueous solutions of IBA increases the rate of rooted plants by 6.2-6.7 % in Vostorg and Krupnoplodnaya varieties when rooting during summer period. Carrying out of one cutting of ex vitro rooted microshoots and further cuttings of obtained material can be used to increase the yield of planting material of blue honeysuckle varieties. Ex vitro rooting and adaptation of blue honeysuckle microshoots at one stage with further ex vitro cutting increases production profitability by 3.5 and 12.9 times in comparison with conventional method of clonal micropropagation of blue honeysuckle.

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The authors declare that there are no conflicts of interest present.