ANALYSIS OF THE GENETIC STABILITY OF IN VITRO PROPAGATED BLUEBERRY (CV. DUKE)
Abstract
The efficiency of propagation methods is of great importance for large-scale production, but even more important is the genetic stability of in vitro propagated planting material. As a result of the research assessed is the possibility of using 54 oligonucleotide primers for the analysis of the genetic stability of in vitro propagated blueberry (cv. Duke) with RAPD-PCR. 6 primers (OPA 01, OPA 10, OPE 3, OPN 14, OL 1, OL 8) producing the maximum number of distinct bands has been selected. It’s established that all RAPD profiles of micropropagated blueberry generated with these 6 primers are monomorphic and identical to those of the mother plant.
About the Authors
T. N. Bozhiday
Институт плодоводства
Russian Federation
Russian Federation
N. N. Volosevich
Институт плодоводства
Russian Federation
Russian Federation
N. V. Kukharchik
Институт плодоводства
Russian Federation
Russian Federation
References
1. Debnath, s. C. Propagation of Vaccinium in vitro: a review / S. C. Debnath // International Journal of Fruit Science. – 2006. – Vol. 6, N 2. – P. 47–71.
2. Debnath, s. C. Propagation and cultivation of Vaccinium species and less known small fruits / S. C. Debnath // Vaccinium ssp. and less known small fruit: challenges and risks: international scientific conference, Jelgava, 6–8 October 2009 / Latvia University of Agriculture; ed.: A. Adamovics [et al.]. – Jelgava, 2009. – P. 22–29.
3. Rani, V. Genetic fidelity of organized meristem-derived micropropagated plants: a critical reappraisal / V. Rani, S. N. Raina // In vitro Cellular and Developmental Biology – Plant. – 2000. – Vol. 36, N 5. – P. 319–330.
4. Высоцкий, В. А. О генетической стабильности при клональном микроразмножении плодовых и ягодных куль-тур / В. А. Высоцкий // Сельскохозяйственная биология. – 1995. – № 5. – С. 57–63.
5. Genetic instability of sugarcane plants derived from meristem cultures / M. I. Zucchi [et al.] // Genetics and Molecular Biology. – 2002. – Vol. 25. – P. 91–96.
6. Assessment of genetic stability and fidelity of some micropropagated Vitis vinifera L. “Feteasca neagra” clones by am-pelometric and RAPD markers / R. N. Gheorghe [et al.] // Bulletin UASVM Horticulture. – 2009. – Vol. 66, N 1. – P. 51–57.
7. Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers / R. Bhatia [et al.] // Plant Cell, Tissue and Organ Culture. – 2011. – Vol. 104, N 1. – P. 131–135.
8. Martins, M. Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers reappraisal / M. Martins, D. Sarmento, M. M. Oliveira // Plant Cell Reports. – 2004. – Vol. 23, N 7. – P. 492–496.
9. Microclonal propagation of Vaccinium sp. and Rubus sp. and detection of genetic variability in culture in vitro / A. Gajdosova [et al.] // Journal of Fruit and Ornamental Plant Research. – 2006. – Vol. 14. – P. 103–119.
10. Государственный реестр сортов / отв. ред.: В. А. Бейня. – Минск, 2014. – С. 83–84, 152–153.
11. Lloyd, G. Commercially-feasible micropropagation of mountain laurel, kalmia latifolia, by use of shoot tip culture / G. Lloyd, B. McCown // Combined Proceedings of the International Plant Propagator’s Society. – 1980. – Vol. 30. – P. 421– 427.
12. Debnath, s. C. In vitro culture of lingonberry (Vaccinium vitis-idaea L.): the influence of cytokinins and media types on propagation / S. C. Debnath, K. B. McRae // Small Fruits Review. – 2001. – Vol. 1, N 3. – P. 3–19.
13. Debnath, s. C. Micropropagation of lingonberry: influence of genotype, explant orientation, and overcoming TDZ-induced inhibition of shoot elongation using zeatin / S. C. Debnath // HortScience. – 2005. – Vol. 40, N 1. – P. 185–188.
14. Debnath, s. C. An assessment of the genetic diversity within a collection of wild cranberry (Vaccinium macrocarpon Ait.) clones with RAPD-PCR / S. C. Debnath // Genetic Resources and Crop Evolution. – 2007. – Vol. 54. – P. 509–517.
15. Пикунова, А. В. Оценка генетического разнообразия исходного и селекционного материала ягодных культур с помощью молекулярных маркеров: дис. … канд. биол. наук: 06.01.05, 03.02.07 / А. В. Пикунова. – Орел, 2011. – 153 л.
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