vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestiag-26Research ArticleЗЕМЛЯРОБСТВА І РАСЛІНАВОДСТВAAGRICULTURE AND PLANT CULTIVATIONАНАЛИЗ ГЕНЕТИЧЕСКОЙ СТАБИЛЬНОСТИ РАСТЕНИЙ ГОЛУБИКИ СОРТА DUKE, ПОЛУЧЕННЫХ В КУЛЬТУРЕ IN VITROANALYSIS OF THE GENETIC STABILITY OF IN VITRO PROPAGATED BLUEBERRY (CV. DUKE)БожидайТ. Н.BozhidayT. N.tanya_bozhidaj@mail.ruВолосевичН. Н.VolosevichN. N.tanya_bozhidaj@mail.ruКухарчикН. В.KukharchikN. V.tanya_bozhidaj@mail.ruИнститут плодоводстваRussian Federation201525052016126063Copyright © Божидай Т.Н., Волосевич Н.Н., Кухарчик Н.В., 20162016Божидай Т.Н., Волосевич Н.Н., Кухарчик Н.В.Bozhiday T.N., Volosevich N.N., Kukharchik N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/26

Эффективность используемых методов размножения имеет большое значение для крупномасштабного производства, однако более важным является получение генетически однородного посадочного материала. В результате проведенных исследований оценена возможность использования 54 олигонуклеотидных праймеров для анализа генетической стабильности растений голубики сорта Duke, полученной в культуре in vitro, с помощью RAPD-PCR и отобраны 6 праймеров (OPA 01, OPA 10, OPE 3, OPN 14, OL 1, OL 8), дающие максимальное количество хорошо различимых полос. Установлено, что отобранные праймеры при амплификации с анализируемыми ДНК-матрицами генерировали идентичные RAPD-спектры, что указывает на отсутствие вариабельности между маточным растением и клонами голубики сорта Duke.

The efficiency of propagation methods is of great importance for large-scale production, but even more important is the genetic stability of in vitro propagated planting material. As a result of the research assessed is the possibility of using 54 oligonucleotide primers for the analysis of the genetic stability of in vitro propagated blueberry (cv. Duke) with RAPD-PCR. 6 primers (OPA 01, OPA 10, OPE 3, OPN 14, OL 1, OL 8) producing the maximum number of distinct bands has been selected. It’s established that all RAPD profiles of micropropagated blueberry generated with these 6 primers are monomorphic and identical to those of the mother plant.

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The authors declare that there are no conflicts of interest present.