THE FIRST EXPERIENCE OF IN VITRO CATTLE EMBRYO PRODUCTION IN THE SYSTEM OF TRANSVAGINAL OOCYTE ASPIRATION (TOA)
Abstract
The results of the first experience of in vitro cattle embryo production in the system of transvaginal oocyte aspiration (TOA) are represented in this article. It’s established that the method of transvaginal oocyte aspiration allows getting 2,7 oocytes per session and 3,3 oocytes per operation. The output of oosytes per session is 2,0 in general. The output of oocytes at blastocyst stage is 8,9 % of the number of matured cells and 21,4 % of the number of divided embryos.
About the Authors
V. K. Pestis
Гродненский государственный аграрный университет
Belarus
Belarus
L. V. Golubets
Гродненский государственный аграрный университет
Belarus
Belarus
A. S. Deshko
Гродненский государственный аграрный университет
Belarus
Belarus
I. S. Kyssa
Гродненский государственный аграрный университет
Belarus
Belarus
M. P. Popov
Гродненский государственный аграрный университет
Belarus
Belarus
Yu. A. Yakubets
Гродненский государственный аграрный университет
Belarus
Belarus
References
1. Callesen, H. Ultrasonically guided aspiration of bovine follicular oocytes / H. Callesen, T. Greve, F. Christensen // Theriogenology. – 1987. – Vol. 27. – P. 217.
2. Effects of aspiration vacuum and needle diameter on cumulus oocyte complex morphology and developmental capacity of bovine oocytes / P. Bols [et al.] // Theriogenology. – 1996. – Vol. 45. – P. 1001–1014.
3. Factors affecting recoverey and quality of oocytes for bovine embryo production in vitro using ovum pickup technology / F. A. Ward [et al.] // Theriogenology. – 2000. – Vol. 54. – P. 433–446.
4. Bols, P. Gebruik van de transvaginale Ovum Pick-Up (OPU) techniek: geboorte van de eerste OPU kalveren in België. (Use of transvaginal oocyte pick-up: first OPU calves bom in Belgium) / P. Bols, A. Soom Van, A. de Kruif // Vlaams Diergeneeskundig Tijdschrift. – 1996. – Vol. 65. – P. 86–91.
5. Boni, r. Impact of Ovum Pick-Up (OPU) technique for research and animal breeding / R. Boni, L. Zicarelli, TAM. Kruip // Reproduction and Animal Breeding: Advancesand Strategy / AETE. – Amsterdam: Elsevier, 1995. – P. 211–221.
6. Follicular dynamics, repeatability and predictability of follicular recruitment in cows submitted to repeated follicular puncture / R. Boni [et al.] // Theriogenology. – 1997. – Vol. 48. – P. 277–289.
7. Effect of the frequency of ovum pick-up intervals on follicle number, oocyte recovery and embryo production rates in cattle / K. Imai [et al.] // Theriogenology. – 2000. – Vol. 53. – P. 359.
8. Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization and culture in vitro / P. Lonergan [et al.] // Mol. Reprod. Dev. – 1994. – Vol. 37. – P. 48–53.
9. Van Soom, A. Improved results in IVF-treatment of sterility patients by careful selection of bulls and by stimulation of cows with FSH before slaughter / A. Van Soom, P. E. J. Bols, A. de Kruif // Reprod. Dom. Anim. – 1995. – Suppl. 3. – P. 67.
10. Gonadotropin stimulation of cattle donors at estrus for transvaginal oocyte collection // J. B. Paul [et al.] // Theriogenology. – 1995. – Vol. 43. – P. 294.
11. Stubbings, r.B. Effect of ultrasonically-guided follicle aspiration on estrous cycle andfollicular dynamics in Holstein cows / R. B. Stubbings, J. S. Walton // Theriogenology. – 1995. – Vol. 43. – P. 705–712.
12. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries / M. C. Pieterse [et al.] // Theriogenology. – 1988. – Vol. 30. – P. 751–762.
13. Transvaginal ultrasound guided follicular aspiration of bovine oocytes / M. C. Pieterse [et al.] // Theriogenology. – 1991. – Vol. 35. – P. 19–24.
14. Repeated transvaginal ultrasound-guided ovum pick-up in ECG-treated cows / M. C. Pieterse [et al.] // Theriogenology. – 1992. – Vol. 37. – P. 273.
15. de ruigh, L. The effect of FSH stimulation prior to ovum pick-up on oocyte and embryo yield / L. De Ruigh, E. Mullaart, AM. van Wagtendonk-de Leeuw // Theriogenology. – 2000. – Vol. 53. – P. 349.
16. Ovum Pick-Up and embryo production in vitro: an established procedure in cattle / R. Boni [et al.] // In: Proceedings of the VIII Congress. European Embryo Transfer Association / AETE. – France. Lyon, 1992. – P. 128.
17. Health and some blood parameters in cows punctured twice weekly during three months to collect immature oocytes / R. Boni [et al.] // In: Proceedings of the IX Congress. European Embryo Transfer Association / AETE. – France. Lyon, 1993. – P. 136.
18. Potential use of Ovum Pick-Up for embryo production and breeding in cattle // T. Kruip [et al.] // Theriogenology. – 1994. – Vol. 42. – P. 675–683.
19. Production, freezing and transfer of bovine IVF embryos and subsequent calving results / J. F. Hasler [et al.] // Theriogenology. – 1995. – Vol. 43. – P. 141–152.
Review
Views: 525
Email this article 