vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestiag-12Research ArticleЖЫВЁЛАГАДОЎЛЯ І ВЕТЭРЫНАРНАЯ МЕДЫЦЫНАANIMAL HUSBANDRY AND VETERINARY MEDICINEПЕРВЫЙ ОПЫТ ПОЛУЧЕНИЯ ЭМБРИОНОВ КРУПНОГО РОГАТОГО СКОТА IN VITRO В СИСТЕМЕ ТРАНСВАГИНАЛЬНОЙ АСПИРАЦИИ ООЦИТОВ (ТАО)THE FIRST EXPERIENCE OF IN VITRO CATTLE EMBRYO PRODUCTION IN THE SYSTEM OF TRANSVAGINAL OOCYTE ASPIRATION (TOA)ПестисВ. К.PestisV. K.ggaubio@mail.ruГолубецЛ. В.GolubetsL. V.ggaubio@mail.ruДешкоА. С.DeshkoA. S.ggaubio@mail.ruКыссаИ. С.KyssaI. S.ggaubio@mail.ruПоповМ. В.PopovM. P.ggaubio@mail.ruЯкубецЮ. А.YakubetsYu. A.ggaubio@mail.ruГродненский государственный аграрный университетBelarus201525052016118691Copyright © Пестис В.К., Голубец Л.В., Дешко А.С., Кысса И.С., Попов М.В., Якубец Ю.А., 20162016Пестис В.К., Голубец Л.В., Дешко А.С., Кысса И.С., Попов М.В., Якубец Ю.А.Pestis V.K., Golubets L.V., Deshko A.S., Kyssa I.S., Popov M.P., Yakubets Y.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/12

Представлены результаты изучения влияния метода получения племенного молодняка в системе трансваги нальной аспирации ооцитов на их извлекаемость и качество. Установлено, что метод трансвагинальной аспирации ооцитов позволяет получить 2,7 ооцит-кумулюсных комплексов за одну аспирацию и 3,3 на одну положительную по извлечению операцию. Выход жизнеспособных ооцит-кумулюсных комплексов составил в целом на одну аспирацию 2,0, в том числе 2,5 на одну положительную по извлечению, извлекаемость – 73,6 %. Выход эмбрионов на стадии бластоциста составил 8,9 % от числа клеток, поставленных на созревание, или 21,4 % от числа дробящихся зародышей.

The results of the first experience of in vitro cattle embryo production in the system of transvaginal oocyte aspiration (TOA) are represented in this article. It’s established that the method of transvaginal oocyte aspiration allows getting 2,7 oocytes per session and 3,3 oocytes per operation. The output of oosytes per session is 2,0 in general. The output of oocytes at blastocyst stage is 8,9 % of the number of matured cells and 21,4 % of the number of divided embryos.

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The authors declare that there are no conflicts of interest present.