Plant-based bacterial starter culture Latilactobacillus sakei

Milk whey is widely used as a main component for a nutrient medium for the production of bacterial starter cultures, but in recent years, bacterial cultures produced from plant-based components have great scientific interest. Lactic acid bacteria strains are often used to create probiotic bacterial cultures. In this regard, the aim of the present study is to develop a technology for the production of a plant-based bacterial starter culture containing Latilactobacillus sakei. The objects of experimental studies were three strains Latilactobacillus sakei, and starter cultures made with the use of these strains. For the cultivation of lactic acid bacteria, MRS medium and a specially developed nutrient medium based on rice flour were used. The growth of Latilactobacillus sakei strains on these media was assessed by the number of viable cells. It has been proved that the new plant-based ingredients nutrient medium allows to obtain bacterial starters with good quality and safety indicators. In terms of safety indicators, they meet the requirements of the Technical Regulation of Customs Union 033/2013 “On the safety of milk and dairy products” and contain a high number of viable cells of lactic acid bacteria (at least 10⁸ CFU/cm³). As a result of the study, new technologies have been developed for the production of liquid and frozen plant-based bacterial starter cultures containing Latilactobacillus sakei strains, which can be used in the production of food products.

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