Intrabreed variability of Landrace animals based on microsatellite DNA polymorphism

The population genetic parameters of Landrace breed animals bred at SE “ZhodinoAgroPlemElita”, PUE “Polesye-Agroinvest” and agricultural branch of SHC “Zadneprovsky” of OJSC “Orshanskiy KHP” were analyzed using microsatellite DNA data. 110 alleles were identified. The number of alleles for the loci ranged from 3 to 10. The highest level of allelic diversity was found in animals bred at SE “ZhodinoAgroPlemElita” – 5.40 alleles per locus. It was revealed that the population of animals of SHC “Zadneprovsky” was in the equilibrium state, the fixation index was equal to 0.001. Deviations from the equilibrium state were observed in pigs of SE “ZhodinoAgroPlemElita” (Fis = –0,019) and PUE “Polesye-Agroinvest” (Fis = –0,018). The actual degree of heterozygosity in animals ranged from 0.549 (PUE “Polesye-Agroinvest”) to 0.662 (SE “ZhodinoAgroPlemElita”). The results of the Hardy-Weinberg equilibrium test showed that most of the MS DNA loci used were in a state of genetic equilibrium. The polymorphism information content (PIC) value of most of the studied loci exceeded 0.5, indicating their high informativeness as molecular genetic markers. The loci S0005 (Fst = 0.103), SW857 (Fst = 0.103) and SW240 (Fst = 0.202) were found to make the most substantial contribution to intrabreed differentiation of Landrace breed pigs. The results of the Assignment test showed that, in general, the accuracy of assignment of the studied animals to “their own” population exceeded 98 %. A high degree of genetic similarity between the Landrace breed animals of these populations was determined, the coefficients of genetic similarity made 0.795–0.874.

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