Factors affecting the level of Fusarium mycotoxins in grain. Vestsі Natsyyanal’nay akademіі navuk Belarusі

Mycotoxins accumulation in grain is one of the most widely analyzed problem nowadays, as soon as these metabolites are of high danger to health of warm-blooded animals and humans. Producing mycotoxins with Fusarium fungi beaing causal agents of Fusarium head blight, depends on range of factors, and among measures for their accumulation decrease, chemical method is one of the most effective ones. The paper presents the results of study on factors affecting mycotoxins accumulation under the field conditions. During the experiment with artificial inoculation of winter wheat heads by F. culmorum fungi, the influence of triazole fungicides on Fusarium head blight and mycotoxins content had been estimated. Higher biological efficiency (83,2-79,2 %) for disease severity decrease was higher with fungicide Osiris, EC. Use of fungicides led to increase in indicators of economic efficiency in comparison with the option with no treatment, including the yield, by 12.3-12.8%. In the variants with head protection, the DON content was 2.0-2.1 times lower than in the control. Under conditions of natural damage to various varieties of grain crops (winter wheat and triticale, spring barley) by Fusarium head blight, its depressive manifestation had been determined. It had been determined that spring barley, regardless of the variety, was more resistant to accumulation of mycotoxins, in particular ZEN. Level of mycotoxins in winter wheat and triticale grain varied depending on variety and storage duration. The data obtained will serve as the basis for substantiating measures to reduce the level of mycotoxins in grain. Acknowledgments. The research was carried out with the financial support of the Belarusian Republican Foundation for Fundamental Research as a part of the scientific research project “Comprehensive assessment of mycotoxins accumulation during foodstuff storage” (treaty N Б19МЛДГ-009).

1. Sedova I. B., Kiseleva M. G., Chalyi Z. A., Aksenov I. V., Zakharova L. P., Tutel’yan V. A. Analysis of the results of monitoring the contamination of food grain with mycotoxins in 2005-2016. Uspekhi meditsinskoi mikologii = Advances in Medical Mycology, 2018, vol. 19, pp. 329-330 (in Russian).

2. Kaminska O. V., Marchenko T. V., Kyryk M. M., Shevchenko L. V. Seasonal dynamics of accumulation of mycotoxins in corn grain. Bіoresursi і prirodokoristuvannya = Biological Resources and Nature Management, 2020, vol. 12, no. 1-2, pp. 47-55 (in Ukrainian). https://doi.org/10.31548/bio2020.01.006

3. Shah L., Ali A., Yahya M., Zhu Y., Wang S., Si H., Rahman H., Ma C. Integrated control of Fusarium head blight and deoxynivalenol mycotoxin in wheat. Plant Pathology, 2018, vol. 67, no. 3, pp. 532-548. https://doi.org/10.1111/ppa.12785

4. Parry D. W., Jenkinson P., McLeod L. Fusarium ear blight (scab) in small grain cereals - a review. Plant Pathology, 1995, vol. 44, no. 2, pp. 207-238. https://doi.org/10.1111/j.1365-3059.1995.tb02773.x

5. Birr T., Hasler M., Verreet J. A., Klink H. Composition and predominance of Fusarium species causing Fusarium head blight in winter wheat grain depending on cultivar susceptibility and meteorological factors. Microorganisms, 2020, vol. 8, no. 4, art. 617. https://doi.org/10.3390/microorganisms8040617

6. Yli-Mattila T., Paavanen-Huhtala S., Parikka P., Konstantinova P., Gagkaeva T. Y. Molecular and morphological diversity of Fusarium species in Finland and northwestern Russia. European Journal of Plant Pathology, 2004, vol. 110, no. 5/6, pp. 573-585. https://doi.org/10.1023/b:ejpp.0000032397.65710.69

7. Stepień Ł., Popiel D., Koczyk G., Chełkowski J. Wheat infecting Fusarium species in Poland - their chemotypes and frequencies revealed by PCR assay. Journal of Applied Genetics, 2008, vol. 49, no. 4, pp. 433-441. https://doi.org/10.1007/bf03195644

8. Wolny-Koładka K., Lenart-Boroń A., Boroń r. Species composition and molecular assessment of the toxigenic potential in the population of Fusarium spp. isolated from ears of winter wheat in southern Poland. Journal of Applied Botany and Food Quality, 2015, vol. 88, no. 1, pp. 139-144.

9. Gagkaeva T. Yu., Gannibal F. B., Gavrilova O. P. Infestation of wheat grain with Fusarium i Alternaria fungi in the south of Russia in 2010. Zashchita i karantin rastenii = Plant Protection and Quarantine, 2012, no. 1, pp. 37-41 (in Russian).

10. Gavrilova O. P., Gagkaeva T. Yu., Burkin A. A., Kononenko G. P. Mycological infection by Fusarium strains and mycotoxins contamination of oats and barley in the north of Nonchernozem’e. Sel’skokhozyaistvennaya biologiya = Agricultural Biology, 2009, vol. 44, no. 6, pp. 89-93 (in Russian).

11. Krupen’ko N. A. Species composition of fungi-causative agents of fusarium ear of winter wheat. Nauchnye stremle niya - 2018: sbornik materialov Mezhdunarodnoi nauchno-prakticheskoi molodezhnoi konferentsii v ramkakh Mezhdunarodnogo nauchno-prakticheskogo innovatsionnogo foruma «INMAX’18» (Minsk, 4-5 dekabrya 2018 g.) [Scientific aspirations - 2018: collection of papers of the International scientific and practical youth conference within the framework of the International scientific and practical innovation forum “INMAX’18” (Minsk, December 4-5, 2018)]. Minsk, 2018, pt. 2, pp. 12-13 (in Russian).

12. Marin D. E., Taranu I., Burlacu R., Tudor D. S. Effects of zearalenone and its derivatives on the innate immune response of swine. Toxicon, 2010, vol. 56, no. 6, pp. 956-963. https://doi.org/10.1016/j.toxicon.2010.06.020

13. Pestka J. J. Deoxynivalenol: mechanisms of action, human exposure, and toxicological relevance. Archives of Toxicology, 2010, vol. 84, no. 9, pp. 663-679. https://doi.org/10.1007/s00204-010-0579-8

14. Capriotti A. L., Foglia P., Gubbiotti R., Roccia C., Samperi R., Laganà A. Development and validation of a liquid chromatography/atmospheric pressure photoionization-tandem mass spectrometric method for the analysis of mycotoxins subjected to commission regulation (EC) No. 1881/2006 in cereals. Journal of Chromatography A, 2010, vol. 1217, no. 39, pp. 6044-6051. https://doi.org/10.1016/j.chroma.2010.07.018

15. Mankevičienė A., Butkutė B., Gaurilčikienė I., Dabkevičius Z., Supronienė S. Risk assessment of Fusarium mycotoxins in Lithuanian small cereal grains. Food Control, 2011, vol. 22, no. 6, pp. 970-976. https://doi.org/10.1016/j.foodcont.2010.12.004

16. Xu X. Effects of environmental conditions on the development of Fusarium ear blight. European Journal of Plant Pathology, 2003, vol. 109, no. 7, pp. 683-689. https://doi.org/10.1023/A:1026022223359

17. Muthomi J. W., Ndung’u J. K., Gathumbi J. K., Mutitu E. W., Wagacha J. M. The occurrence of Fusarium species and mycotoxins in Kenyan wheat. Crop Protection, 2008, vol. 27, no. 8, pp. 1215-1219. https://doi.org/10.1016/j.cropro.2008.03.

18. Chekmaryev V. V., Kobylskaya G. V., Buchneva G. N., Korabelskaya O. I. Forecasting the Fusarium blight infec tions of winter wheat seeds. Zashchita i karantin rastenii = Plant Protection and Quarantine, 2012, no. 1, pp. 41-43 (in Russian).

19. O’Donnell K., Ward T. J., Aberra D., Kistler H. C., Aoki T., Orwig N., Kimura M., Bjørnstad Å., Klemsdal S. S. Multilocus genotyping and molecular phylogenetics resolve a novel head blight pathogen within the Fusarium graminearum species complex from Ethiopia. Fungal Genetics and Biology, 2008, vol. 45, no. 11, pp. 1514-1522. https://doi.org/10.1016/j.fgb.2008.09.002

20. Toropova E. Yu., Vorob’eva I. G., Mustafina M. A., Selyuk M. P. Fusarium link. Fungi on the wheat grains in Western Siberia. Agrokhimiya = Agricultural Chemistry, 2019, no. 5, pp. 76-82 (in Russian). https://doi.org/10.1134/S0002188119050119

21. L’vova L. S., Kizlinko O. I., Shulgina A. P., Omelchenko M. D., Zakharova L. P., Piminova V. V., Gagkaeva T. Yu. Peculiarities of dezoxinivalenol and zearalenon synthesis in wheat grain infected by fusariosis of spike. Mikologiya i fitopatologiya = Mycology and Phytopathology, 1997, vol. 31, iss. 6, pp. 52-58 (in Russian).

22. Chulze S. N., Palazzini J. M., Torres A. M., Barros G., Ponsone M. L., Geisen R., Schmidt-Heydt M., Köhl J. Biological control as a strategy to reduce the impact of mycotoxins in peanuts, grapes and cereals in Argentina. Food Additives and Contaminants. Part A, 2015, vol. 32, no. 4, pp. 471-479. https://doi.org/10.1080/19440049.2014.984245

23. Wegulo S. N., Baenziger P. S., Nopsa J. H., Bockus W. W., Hallen-Adams H. Management of Fusarium head blight of wheat and barley. Crop Protection, 2015, vol. 73, pp. 100-107. https://doi.org/10.1016/j.cropro.2015.02.025

24. Pirgozliev S. R., Edwards S. G., Hare M. C., Jenkinson P. Effect of dose rate of azoxystrobin and metconazole on the development of Fusarium head blight and the accumulation of deoxynivalenol (DON) in wheat grain. European Journal of Plant Pathology, 2002, vol. 108, no. 5, pp. 469-478. https://doi.org/10.1023/A:1016010812514

25. Klix M. B., Verreet J.-A., Beyer M. Comparison of the declining triazole sensitivity of Gibberella zeae and increased sensitivity achieved by advances in triazole fungicide development. Crop Protection, 2007, vol. 26, no. 4, pp. 683-690. https://doi.org/10.1016/j.cropro.2006.06.006

26. Paul P. A., Lipps P. E., Hershman D. E., McMullen M. P., Draper M. A., Madden L. V. Efficacy of triazole-based fungicides for Fusarium head blight and deoxynivalenol control in wheat: a multivariate meta-analysis. Phytopathology, 2008, vol. 98, no. 9, pp. 999-1011. https://doi.org/10.1094/PHYTO-98-9-0999

27. Haidukowski M., Pascale M., Perrone G., Pancaldi D., Campagna C., Visconti A. Effect of fungicides on the development of Fusarium head blight, yield and deoxynivalenol accumulation in wheat inoculated under field conditions with Fusarium graminearum and Fusarium culmorum. Journal of the Science of Food and Agriculture, 2005, vol. 85, no. 2, pp. 191-198. https://doi.org/10.1002/jsfa.1965

28. Simpson D. R., Weston G. E., Turner J. A., Jennings P., Nicholson P. Differential control of head blight pathogens of wheat by fungicides and consequences for mycotoxin contamination of grain. European Journal of Plant Pathology, 2001, vol. 107, pp. 421-431. https://doi.org/10.1023/A:1011225817707

29. Ramirez M. L., Chulze S. N., Magan M. Impact of environmental factors and fungicides on growth and deoxynivalenol production by Fusarium graminearum isolates from Argentinian wheat. Crop Protection, 2004, vol. 23, no. 2, pp. 117-125. https://doi.org/10.1016/j.cropro.2003.07.005

30. Boyacioglu D., Hettiarachchy N. S., Stack R. W. Effect of three systemic fungicides on deoxynivalenol (vomitoxin) production by Fusarium graminearum in wheat. Canadian Journal of Plant Science, 1992, vol. 72, no. 1, pp. 93-101.

31. Bala B. K. Drying and storage of cereal grains. 2nd ed. Chichester, Wiley Blackwell, 2016. 352 p.

32. Orina A. S., Gavrilova O. P., Gagkaeva T. Y ., Gogina N. N. Contamination of grain in West Siberia by Alternaria fungi and their mycotoxins. Vestnik zashchity rastenii = Plant Protection News, 2021, vol. 104, no. 3, pp. 153-162 (in Russian). https://doi.irg/10.31993/2308-6459-2021-104-3-15019

33. Buga S. F., Artiomova O. V., Iliuk A. G. Pathogenesis reculiarities of winter wheat (Triticum aestivum Z.) ear at inoculation of Fusarium spp. Fungi. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya agrarnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Agrarian series, 2005, no. 4, pp. 71-76 (in Russian).

34. Il’yuk A. G., Buga S. F. The harmfulness of fusarium spike of winter wheat. Zashchita rastenii: sbornik nauchnykh trudov = Plant protection: collection of scientific papers. Minsk, 2006, iss. 30, pt. 1, pp. 227-230 (in Russian).

35. Tateishi H., Miyake T., Mori M., Sakuma Y., Saishoji T. Effect of application timing of metconazole on Fusarium head blight development and mycotoxin contamination in wheat and barley. Journal of Pesticide Science, 2014, vol. 39, no. 1, pp. 1-6. https://doi.org/10.1584/jpestics.D12-077