Amino-acid composition and balance of collagen containing raw materials

A prospective source of additional food protein in the meat industry is collagen-containing raw materials: pork skin, connective tissue obtained at meat trimming, collagen-containing by-products, etc., which can be used as part of proteinand-fat emulsions. Collagen emulsions have a wide range of functional properties: moisture and fat retention, foam and gelling abilities, emulsifying activity, active foam, emulsions and dispersions stabilizers, so they can be used as a technological additive in the meat industry. Therefore, the most important task in the process is the complete use of collagen-containing raw materials with the maximum implementation of its properties. The paper presents the results of studies on determining the amino acid composition and balance of collagen-containing raw materials: pork skin, beef tendons, lungs, rumen, beef lips and ears, pork ears, udder, spleen, omasum, skin, head, feet, caruncle, stomach and intestines of poultry. It was determined that collagen-containing raw material contains all the essential amino acids: isoleucine, leucine, lysine, methionine and cysteine, phenylalanine and tyrosine, threonine, valine with the amino acid count in the range of 17.1–185.0 %. At the same time, a more balanced amino acid composition in comparison with other types of collagen-containing raw materials is shown by: pork skin, udder, beef lips and trachea, chitterlings, omasum, rumen, lungs and spleen as evidenced by high values of amino acid score (up to 185 %), essential amino acid index (up to 1.1), utility factor of the amino acid composition (up to 0.84), as well as the approximate values of the comparable redundancy index (0.0007–0.0043). The research results confirm the prospectiveness of combining muscle and connective tissue proteins in recipes of meat products of high nutritional and biological value.

collagen-containing raw materials, essential amino acids, amino acid score, essential amino acid index, amino acid composition utility ratio, comparable redundancy indicator

1. Antipova L. V., Glotova I. A. Use of secondary collagen containing raw materials of meat industry. St. Petersburg, GIORD Publ., 2006. 383 p. (in Russian).

2. Antipova L. V., Storublevtsev S. A. Prospects of using by-products of farm animals slaughter for food and obtaining collagen substances. Agrarnaya nauka i obrazovanie na sovremennom etape razvitiya: opyt, problemy i puti ikh resheniya: materialy mezhdunarodnoi nauchno-prakticheskoi konferentsii, Ul'yanovsk, 26–28 maya 2009 g. [Agrarian science and education at the current stage of development: experience, problems and solutions: materials of the international scientific and practical conference, Ulyanovsk, May 26–28, 2009]. Ulyanovsk, 2009, vol. 2, pp. 151–153 (in Russian).

3. Artem'eva I. O. The use of composites based on modified collagen in the technology of meat products production. Myasnye tekhnologii [Meat Technology], 2017, no. 3, pp. 40–42 (in Russian).

4. Bitueva E. B., Zhamsaranova S. D. Elastin and prospects of its use in food technology with special properties. Khranenie i pererabotka sel'khozsyr'ya = Storage and Processing of Farm Products, 2004, no. 2, pp. 47–49 (in Russian).

5. Budaeva A. E., Bazhenova B. A., Danilov A. M. Chemical modification of collagen containing raw materials for use in production of meat products. Vse o myase [All about meat], 2015, no. 1, pp. 31–35 (in Russian).

6. Volik V. G., Ismailova D. Yu., Zinov'ev S. V., Erokhina O. N. Collagen containing protein for food industry. Ptitsa i ptitseprodukty = Poultry & Chicken Products, 2017, no. 6, pp. 61–64 (in Russian).

7. Volik V. G., Ismailova D. Yu., Zinov'ev S. V., Erokhina O. N. Modern technologies of processing secondary rawmaterials of meat and piper processing industry. Krolikovodstvo i zverovodstvo [Rabbit Breeding and Fur-Bearing Animal Breeding], 2017, no. 3, pp. 11–15 (in Russian).

8. Gushchin V. V., Sokolova L. A. Possibility of unconventional use of some low-value products in industrial poultry processing. Ptitsa i ptitseprodukty = Poultry & Chicken Products, 2009, no. 6, pp. 29–30 (in Russian).

9. Zharinov A. I., Voyakin M. P. (ed.). Short trainings on the bases of modern meat processing technologies, organized by the company "Protein Technologies International" (USA). Pt. 2. Musculoskeletal and restructured meat products. Moscow, 1994. 179 p. (in Russian).

10. Kashchenko R. V. Development of the method for enzymatic treatment of collagen containing raw materials and its application in the technology of cooked sausages. Ph.Dr. thesis in engineering. Moscow, 2007. 178 p. (in Russian).

11. Apraksina S. K., Kashchenko R. V. Increase of nutritional adequacy of collagen containing raw materials through enzymatic treatment. Vse o myase [All about meat], 2006, no. 4, pp. 11–12 (in Russian).

12. Lukin A. A., Merenkova S. P. A method for producing protein stabilizer for sausage. Tekhnologiya i tovarovedenie innovatsionnykh pishchevykh produktov = Technology and Merchandising of the Innovative Foodstuff, 2016, no. 1, pp. 15–19 (in Russian).

13. Martynov G. V., Litvinova E. V. Use of biomodified collagen-containing raw materials in the technology of pork products. Myasnye tekhnologii [Meat Technology], 2016, no. 9, pp. 85–88 (in Russian).

14. Ushakova I. A. Use of modified tripe in the production of chopped meat semi-products. Ph.Dr. thesis in engineering. Moscow, 1998. 116 p. (in Russian).

15. Kishen'ko I. I., Topchii O. A. Protein-in-oil emulsion and its effect on the quality of salted meat products. Innovatsionnye tekhnologii v pishchevoi promyshlennosti: materialy X mezhdunarodnoi nauchno-prakticheskoi konferentsii, Minsk, 5–6 oktyabrya 2011 g. [Innovative technologies in food industry: materials of the Xth International scientific and practical conference, Minsk, 5–6 October 2011]. Minsk, 2011, pt. 2, pp. 164–171 (in Russian).

16. Sokolov A. Yu., Titov A. I., Apraksina S. K., Litvinova E. V. Microstructural and rheological properties of collagencontaining raw material upon its modification. Myasnaya industriya = Meat Industry, 2016, no. 6, pp. 43–45 (in Russian).

17. Stanovova I. A., Vostrikova N. L., Kurzova A. A., Kuznetsova T. G., Nasonova V. V. Identification of the content of collagen in animal proteins. Vse o myase [All about meat], 2017, no. 3, pp. 11–13 (in Russian).

18. Stepantsova G. E., Sergeeva N. T., Vorob'ev V. I. Bioactive substances of collagen containing raw materials and their physiological value. Izvestiya KGTU = KSTU News, 2010, no. 18, pp. 118–124 (in Russian).

19. Titov E. I., Apraksina S. K., Vasil'eva I. O., Sokolov A. Yu., Emel'yanenko V. I., Grishchenko V. M. The collagen matrix for immobilization of biologically active substances. Khranenie i pererabotka sel'khozsyr'ya = Storage and Processing of Farm Products, 2013, no. 3, pp. 39–44 (in Russian).

20. Lebedeva L. I., Nasonova V. V., Verevkina M. I. Methods for processing of collagen containing raw materials and by-products. Myasnaya industriya = Meat Industry, 2016, no. 4, pp. 44–47 (in Russian).