vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2024-62-2-114-125vestiag-748Research ArticleЗЕМЛЯРОБСТВА І РАСЛІНАВОДСТВAAGRICULTURE AND PLANT CULTIVATIONГлифосат и аминометилфосфоновая кислота в природных средах и их микробная трансформацияGliphosate and aminomethylphosphonic acid in the environment and their microbial transformationМихайловскаяН. А.MikhailouskayaN. A.

Михайловская Наталья Алексеевна – кандидат сельскохозяйственных наук, доцент, заведующий лабораторией

ул. Казинца, 90, 220108, Минск

Natallia A. Mikhailouskaya – Ph. D. (Agriculture), Associate Professor, Head of the Laboratory

90, Kazinets Str., 220108, Minsk

bionf1@yandex.ruИнститут почвоведения и агрохимии, Национальная академия наук БеларусиInstitute of Soil Science and Agrochemistry, National Academy of Sciences of Belarus202427042024622114125Copyright © Михайловская Н.А., 20242024Михайловская Н.А.Mikhailouskaya N.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/748

Анализ отечественной и зарубежной научной литературы свидетельствует, что глобальное применение глифосата (ГФ), обусловленное его эффективностью, невысокой стоимостью и созданием устойчивых к гербициду сортов сельскохозяйственных культур, привело к практически повсеместному присутствию остаточных количеств гербицида и его метаболита, аминометилфосфоновой кислоты (АМФК), в окружающей среде: в воздухе, почвах, природных водах и в продукции растениеводства. Представлена научная информация по влиянию глифосата на окружающую среду и живые организмы. Обоснована необходимость периодической ремедиации для снижения негативных последствий многократного применения гербицида и детоксикации его остатков с применением бактерий-деструкторов, способных разлагать глифосат и АМФК до экологически безопасных соединений. Рассмотрены пути микробной трансформации глифосата. Безопасная детоксикация предполагает применение бактериальных деструкторов, разрушающих фосфоновую связь в молекуле ГФ. Несмотря на то что способность к биодеградации глифосата проявляют бактерии разных родов, коммерческие препараты для безопасной детоксикации ГФ пока не разработаны из-за высокого уровня штаммовой специфичности, связанной с различием путей катаболизма ГФ. Наиболее перспективны поиски деструкторов ГФ и АМФК среди ризосферных бактерий, предназначенных для применения в качестве инокулянтов. Сложность проблемы детоксикации ГФ и АМФК и высокий уровень штаммовой специфичности бактериальных деструкторов сдерживают разработку коммерческих препаратов для детоксикации ГФ и АМФК.

Analysis of domestic and international literature indicates that the global use of glyphosate (GP), due to its effectiveness, low price and creation of herbicide-resistant agricultural crop varieties, resulted in nearly universal presence of the rest quantities of the herbicide and its main metabolite, aminomethylphosphonic acid (АМPA) in the environment: air, soil, water and crop products. Scientific information on glyphosate influence on the environment and living organisms is presented in this paper. The necessity is substantiated for periodic remediation for reducing the negative consequences of the repeated application of herbicide and detoxification of its residual quantities with the use of destructor-bacteria, capable of decomposing glyphosate and AMPA to ecologically safe compounds. The ways for glyphosate microbial transformation are reviewed. Ecologically safe detoxification assumes the use of bacterial destructors, which are destroying the phosphonic bond in glyphosate molecule. Although the bacteria of different genera are showing capacity for GP biodegradation, commercial products for the safe detoxification of glyphosate have not yet been developed due to the high level of strain specificity associated with the different ways of GF catabolism. The most perspective is the search for GP and AMPA destructors among rhizosphere bacteria, intended for application as inoculants. Complexity of the problem of GP and AMPA detoxification as well as the high level of strain specificity of bacterial destructors significantly restrain development of commercial preparations for GP and AMPA detoxification.

глифосат (ГФ)аминометилфосфоновая кислота (АМФК)микробная деструкциябактерии- деструкторы ГФ и АМФКпути катаболизмаштаммовая специфичностьдетоксикация ГФ и АМФКglyphosate (GP)aminomethylphosphonic acid (АМPA)microbial destructiondestructor-bacteria of GP and AMPAcatabolism waysstrain specificitydetoxification of GP and АМPReferencesDuke, S. O. Glyphosate: a once-in-a-century herbicide / S. O. Duke, S. B. Powles // Pest Manag. Sci. – 2008. – Vol. 64, № 4. – Р. 319–325. https://doi.org/10.1002/ps.1518Duke S. O., Powles S. B. Glyphosate: a-once-in-a century herbicide. Pest Management Science, 2008, vol. 64, no. 4, pp. 319–325. https://doi.org/10.1002/ps.1518Carlisle, S. M. Glyphosate in the environment / S. M. Carlisle, J. T. Trevors // Water, Air Soil Pollut. – 1988. – Vol. 39. – P. 409–420. https://doi.org/10.1007/BF00279485Carlisle S. M., Trevors J. T. Glyphosate in the environment. Water, Air and Soil Pollution, 1988, vol. 39, pp. 409–420. https://doi.org/10.1007/BF00279485Recent advances in glyphosate biodegradation / H. Zhan [et al.] // Appl. Microbiol. Biotechnol. – 2018. – Vol. 102, № 12. – P. 5033–5043. https://doi.org/10.1007/s00253-018-9035-0Zhan H., Feng Y., Fan X., Chen S. Recent advances in glyphosate biodegradation. Applied Microbiology and Biotechnology, 2018, vol. 102, no. 12, pp. 5033–5043. https://doi.org/10.1007/s00253-018-9035-0Кононова, С. В. Фосфонаты и их деградация микроорганизмами / С. В. Кононова, М. А. Несмеянова // Биохимия. – 2002. – Т. 67, вып. 2. – С. 220–233.Kononova S. V., Nesmeyanova M. A. Phosphonates and their degradation by microorganisms. Biokhimiya [Biochemistry], 2002, vol. 67, no. 2, pp. 220–233 (in Russian).Borggaard, O. K. Fate of glyphosate in soil and the possibility of leaching to ground and surface waters: a review / O. K. Borggaard, A. L. Gimsing // Pest Manag. Sci. – 2008. – Vol. 64, № 4. – P. 441–456. https://doi.org/10.1002/ps.1512Borggaard O. K., Gimsing A. L. Fate of glyphosate in soil and the possibility of leaching to ground and surface waters: a review. Pest Management Science, 2008, vol. 64, no. 4, pp. 441–456. https://doi.org/10.1002/ps.1512Bai, S. H. Glyphosate: environmental contamination, toxicity and potential risks to human health via food contamination / S. H. Bai, S. M. Ogbourne // Environ. Sci. Pollut. Res. – 2016. – Vol. 23, № 19. – P. 18988–19001. https://doi.org/10.1007/s11356-016-7425-3Bai S. H., Ogbourne S. M. Glyphosate: environmental contamination, toxicity and potential risks to human health via food contamination. Environmental Science and Pollution Research, 2016, vol. 23, no. 19, pp. 18988–19001. https://doi.org/10.1007/s11356-016-7425-3Occurrence of glyphosate and AMPA in an agricultural watershed from the southeastern region of Argentina / L. Lupi [et al.] // Sci. Total Environ. – 2015. – Vol. 536. – Р. 687–694. https://doi.org/10.1016/j.scitotenv.2015.07.090Lupi L., Miglioranza K. S. B., Aparicio V. C., Marino D., Bedmar F., Wunderlin D. A. Occurrence of glyphosate and AMPA in an agricultural watershed from the southeastern region of Argentina. Science of the Total Environment, 2015, vol. 536, pp. 687–694. https://doi.org/10.1016/j.scitotenv.2015.07.090Жариков, М. Г. Эколого-токсикологическая оценка многолетнего применения глифосата на дерново-подзолистой почве и биоремедиация загрязненных территорий: дис. … канд. биол. наук: 03.02.08 / М. Г. Жариков. – М., 2012. – 118 л.Zharikov M. G. Ecological and toxicological assessment of long-term use of glyphosate on sod-podzolic soil and bioremediation of contaminated areas. Moscow, 2012. 118 p. (in Russian).Vereecken, H. Mobility and leaching of glyphosate: a review / H. Vereecken // Pest Manag. Sci. – 2005. – Vol. 61, № 12. – P. 1139–1151. https://doi.org/10.1002/ps.1122Vereecken H. Mobility and leaching of glyphosate: a review. Pest Management Science, 2005, vol. 61, no. 12, pp. 1139–1151. https://doi.org/10.1002/ps.1122Glyphosate persistence in seawater / P. Mercurio [et al.] // Mar. Pollut. Bull. – 2014. – Vol. 85, № 2. – Р. 385–390. https://doi.org/10.1016/j.marpolbul.2014.01.021Mercurio P., Flores F., Mueller J. F., Carter S., Negri A. P. Glyphosate persistence in seawater. Marine Pollution Bulletin, 2014, vol. 85, no. 2, pp. 385–390. https://doi.org/10.1016/j.marpolbul.2014.01.021Annett, R. Impact of glyphosate and glyphosate-based herbicides on the freshwater environment / R. Annett, H. R. Habibi, A. Hontela // J. Appl. Toxicol. – 2014. – Vol. 34, № 5. – Р. 458–479. https://doi.org/10.1002/jat.2997Annett R., Habibi H. R., Hontela A. Impact of glyphosate and glyphosate-based herbicides on the freshwater environment. Journal of Applied Toxicology, 2014, vol. 34, no. 5, pp. 458–479. https://doi.org/10.1002/jat.2997Grandcoin, A. AminoMethylPhosphonic acid (AMPA) in nature waters: its sources, behavior and environmental fate / A. Grandcoin, S. Piel, E. Baures // Water Res. – 2017. – Vol. 117. – P. 187–197. https://doi.org/10.1016/j.watres.2017.03.055Grandcoin A., Piel S., Baures E. AminoMethylPhosphonic acid (AMPA) in nature waters: its sources, behavior and environmental fate. Water Research, 2017, vol. 117, pp. 187–197. https://doi.org/10.1016/j.watres.2017.03.055Relevance of urban glyphosate use for surface water quality / I. Hanke [et al.] // Chemosphere. – 2010. – Vol. 81, № 3. – P. 422–429. https://doi.org/10.1016/j.chemosphere.2010.06.067Hanke I., Wittmer I., Bischofberger S., Stamm C., Singer H. Relevance of urban glyphosate use for surface water quality. Chemosphere, 2010, vol. 81, no. 3, pp. 422–429. https://doi.org/10.1016/j.chemosphere.2010.06.067Transfer of glyphosate and its degrade AMPA to surface waters through urban sewerage systems / F. Botta [et al.] // Chemosphere. – 2009. – Vol. 77, № 1. – P. 133–139. https://doi.org/10.1016/j.chemosphere.2009.05.008Botta F., Lavison G., Couturier G., Alliot F., Moreau-Guigon E., Fauchon N., Guery B., Chevreuil M., Blanchoud H. Transfer of glyphosate and its degrade AMPA to surface waters through urban sewerage systems. Chemosphere, 2009, vol. 77, no. 1, pp. 133–139. https://doi.org/10.1016/j.chemosphere.2009.05.008Glyphosate and AMPA in Drinking-water: background doc. for development of WHO Guidelines for Drinking-water Quality / World Health Organization. – Geneva: WHO, 2005. – 10 p.Glyphosate and AMPA in drinking-water: background document for development of WHO Guidelines for Drinking-water Quality. Geneva, World Health Organization, 2005. 10 p.Шувалова, Н. Е. Биотехнологические аспекты определения токсичности пестицидов на клеточных и организменных тест-системах: дис. … канд. биол. наук: 1.5.6 / Н. Е. Шувалова. – Тверь, 2021. – 137 л.Shuvalova N. E. Biotechnological aspects of pesticide toxicity determination on cellular and organismal test systems. Tver, 2021. 137 p. (in Russian).Glass, R. L. Adsorption of glyphosate by soils and clay minerals / R. L. Glass // J. Agric. Food Chem. – 1987. – Vol. 35, № 4. – Р. 497–500. https://doi.org/10.1021/jf00076a013Glass R. L. Adsorption of glyphosate by soils and clay minerals. Journal of Agricultural and Food Chemistry, 1987, vol. 35, no. 4, pp. 497–500. https://doi.org/10.1021/jf00076a013Gimsing, A. L. Competitive adsorption and desorption of glyphosate and phosphate on clay silicates and oxides / A. L. Gimsing, O. K. Borggaard // Clay Minerals. – 2002. – Vol. 37, № 3. – P. 509–515. https://doi.org/10.1180/0009855023730049Gimsing A. L., Borggaard O. K. Competitive adsorption and desorption of glyphosate and phosphate on clay silicates and oxides. Clay Minerals, 2002, vol. 37, no. 3, pp. 509–515. https://doi.org/10.1180/0009855023730049Glyphosate sorption in soils of different pH and phosphorus content / H. de Jonge [et al.] // Soil Sci. – 2001. – Vol. 166, № 4. – Р. 230–238. https://doi.org/10.1097/00010694-200104000-00002Jonge H. de, Jonge L. W. de, Jacobsen O. H., Yamaguchi T., Moldrup P. Glyphosate sorption in soils of different pH and phosphorus content. Soil Science, 2001, vol. 166, no. 4, pp. 230–238. https://doi.org/10.1097/00010694-200104000-00002The influence of organic matter on sorption and fate of glyphosate in soil – comparing different soils and humic substances / C. N. Albers [et al.] // Environ. Pollut. – 2009. – Vol. 157, № 10. – P. 2865–2870. https://doi.org/10.1016/j.envpol.2009.04.004Albers C. N., Banta G. T., Hansen P. E., Jacobsen O. S. The influence of organic matter on sorption and fate of glyphosate in soil – comparing different soils and humic substances. Environmental Pollution, 2009, vol. 157, no. 10, pp. 2865– 2870. https://doi.org/10.1016/j.envpol.2009.04.004Шушкова, Т. В. Биодеструкция глифосата почвенными бактериями: дис. … канд. биол. наук: 03.01.06 / Т. В. Шуш-кова. – Пущино, 2010. – 128 л.Shushkova T. V. Biodegradation of glyphosate by soil bacteria. Pushchino, 2010. 128 p. (in Russian).Микробная деградация гербицида глифосата (обзор) / А. В. Свиридов [и др.] // Приклад. биохимия и микробиология. – 2015. – Т. 51, вып. 2. – С. 183–190. https://doi.org/10.7868/S0555109915020221Sviridov A. V., Shushkova T. V., Ermakova I. T., Ivanova E. V., Epiktetov D. O., Leontievsky A. A. Microbial degradation of glyphosate herbicides (review). Applied Biochemistry and Microbiology, 2015, vol. 51, no. 2, pp. 188–195. https://doi.org/10.1134/s0003683815020209Биодеградация фосфорорганических загрязнителей почвенными бактериями: биохимические аспекты и нерешенные проблемы / А. В. Свиридов [и др.] // Биотехнология. – 2020. – Т. 36, № 4. – С. 126–135. https://doi.org/10.21519/0234-2758-2020-36-4-126-135Sviridov A. V., Shushkova T. V., Epiktetov D. O., Tarlachkov S. V., Ermakova I. T., Leontievskii A. A. Biodegradation of organophosphorus pollutants by soil bacteria: biochemical aspects and unsolved problems. Biotekhnologiya = Biotechnology, 2020, vol. 36, no. 4, pp. 126–135 (in Russian). https://doi.org/10.21519/0234-2758-2020-36-4-126-135Ермакова, И. Т. Микробная деструкция органофосфонатов почвенными бактериями / И. Т. Ермакова, Т. В. Шушкова, А. А. Леонтьевский // Микробиология. – 2008. – Т. 77, № 5. – С. 689–695.Ermakova I. T., Shushkova T. V., Leont’evskii A. A. Microbial degradation of organophosphonates by soil bacteria. Microbiology, 2008, vol. 77, no. 5, pp. 615–620. https://doi.org/10.1134/s0026261708050160Al-Rajab, A. J. Degradation of 14C-glyphosate and aminomethylphosphonic acid (AMPA) in three agricultural soils / A. J. Al-Rajab, M. Schiavon // J. Environ. Sci. – 2010. – Vol. 22, № 9. – P. 1374–1380. https://doi.org/10.1016/s1001-0742(09)60264-3Al-Rajab A. J., Schiavon M. Degradation of 14C-glyphosate and aminomethylphosphonic acid (AMPA) in three agricultural soils. Journal of Environmental Sciences, 2010, vol. 22, no. 9, pp. 1374–1380. https://doi.org/10.1016/s1001-0742(09)60264-3Effects of repeated glyphosate applications on soil microbial community composition and the mineralization of glyphosate / S. H. Lancaster [et al.] // Pest Manag. Sci. – 2010. – Vol. 66, № 1. – P. 59–64. https://doi.org/10.1002/ps.1831Lancaster S. H., Hollister E. B., Senseman S. A., Gentry T. J. Effects of repeated glyphosate applications on soil microbial community composition and the mineralization of glyphosate. Pest Management Science, 2010, vol. 66, no. 1, pp. 59–64. https://doi.org/10.1002/ps.1831Сорбция глифосата и его микробная деградация в почвенных суспензиях / Т. В. Шушкова [и др.] // Приклад. биохимия и микробиология. – 2009. – Т. 45, № 6. – С. 664–669.Shushkova T. V., Ermakova I. T., Leontievsky A. A., Vasilieva G. K. Sorption and microbial degradation of glyphosate in soil suspensions. Applied Biochemistry and Microbiology, 2009, vol. 45, no. 6, pp. 599–603. https://doi.org/10.1134/s0003683809060040Ratcliff, A. W. Changes in microbial community structure following herbicide (glyphosate) additions to forests soils / A. W. Ratcliff, M. D. Busse, C. J. Shestak // Appl. Soil Ecol. – 2006. – Vol. 34, № 2–3. – P. 114–124. https://doi.org/10.1016/j.apsoil.2006.03.002Ratcliff A. W., Busse M. D., Shestak C. J. Changes in microbial community structure following herbicide (glyphosate) additions to forests soils. Applied Soil Ecology, 2006, vol. 34, no. 2–3, pp. 114–124. https://doi.org/10.1016/j.apsoil.2006.03.002Glyphosate toxicity and the effects of long term vegetation control on soil microbial communities / M. Busse [et al.] // Soil Biol. Biochem. – 2001. – Vol. 33, № 12–13. – P. 1777–1789. https://doi.org/10.1016/S0038-0717(01)00103-1Busse M., Ratcliff A. W., Shestak C. J., Powers R. F. Glyphosate toxicity and the effects of long term vegetation control on soil microbial communities. Soil Biology and Biochemistry, 2001, vol. 33, no. 12–13, pp. 1777–1789. https://doi.org/10.1016/S0038-0717(01)00103-1Железова, А. Д. Изменение функциональных и структурных характеристик прокариотного сообщества почв под воздействием гербицида глифосата: автореф. дис. … канд. биол. наук: 03.02.03 / А. Д. Железова. – М., 2018. – 20 с.Zhelezova A. D. Changes in the functional and structural characteristics of the prokaryotic community of soils under the influence of the herbicide glyphosate. Moscow, 2018. 20 p. (in Russian).Glyphosate suppresses the antagonistic effect of Enterococcus spp. on Clostridium botulinum / M. Krüger [et al.] // Anaerobe. – 2013. – Vol. 20. – P. 74–78. https://doi.org/10.1016/j.anaerobe.2013.01.005Krüger M., Shehata A. A., Schrödl W., Rodloff A. Glyphosate suppresses the antagonistic effect of Enterococcus spp. on Clostridium botulinum. Anaerobe, 2013, vol. 20, pp. 74–78. https://doi.org/10.1016/j.anaerobe.2013.01.005Zablotowicz, R. M. Impact of glyphosate on Bradyrhizobium japonicum symbiosis with glyphosate-resistant transgenic soybean: а minireview / R. M. Zablotowicz, K. N. Reddy // J. Environ. Qual. – 2004. – Vol. 33, № 3. – Р. 825–831. https://doi.org/10.2134/jeq2004.0825Zablotowicz R. M., Reddy K. N. Impact of glyphosate on Bradyrhizobium japonicum symbiosis with glyphosate-resistant transgenic soybean: а minireview. Journal of Environmental Quality, 2004, vol. 33, no. 3, pp. 825–831. https://doi.org/10.2134/jeq2004.0825Sannino, F. Pesticide influence on soil enzymatic activities / F. Sannino, L. Gianfreda // Chemosphere. – 2001. – Vol. 45, № 4–5. – Р. 417–445. https://doi.org/10.1016/s0045-6535(01)00045-5Sannino F., Gianfreda L. Pesticide influence on soil enzymatic activities. Chemosphere, 2001, vol. 45, no. 4–5, pp. 417–445. https://doi.org/10.1016/s0045-6535(01)00045-5Haslam, E. The shikimate pathway: biosynthesis of natural products series / E. Haslam. – London: Butterworths, 1974. – 316 p. https://doi.org/10.1016/C2013-0-04212-0Haslam E. The shikimate pathway: biosynthesis of natural products series. London, Butterworths, 1974. 316 p. https://doi.org/10.1016/C2013-0-04212-0Chaufan, G. Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: differences with its active ingredient / G. Chaufan, I. Coalova, M. de Molina // Int. J. Toxicol. – 2014. – Vol. 33, № 1. – Р. 29–38. https://doi.org/10.1177/1091581813517906Chaufan G., Coalova I., Molina M. de. Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: differences with its active ingredient. International Journal of Toxicology, 2014, vol. 33, no. 1, pp. 29–38. https://doi.org/10.1177/1091581813517906Benachour, N. Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells / N. Benachour, G. Séralini // Chem. Res. Toxicol. – 2009. – Vol. 22, № 1. – Р. 97–105. https://doi.org/10.1021/tx800218nBenachour N., Seralini G. Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells. Chemical Research in Toxicology, 2009, vol. 22, no. 1, pp. Р. 97–105. https://doi.org/10.1021/tx800218nSéralini, G. Differential Effects of Glyphosate and Roundup on Human Placental Cells and Aromatase / G. Séralini, S. Moslemi // Molecular and Cellular Endocrinology. − 2018. − Vol. 178 (1–2). − P. 117–131. https://doi.org/10.1016/S0303-7207(01)00433-6Séralini G., Moslemi S. Differential Effects of Glyphosate and Roundup on Human Placental Cells and Aromatase. Molecular and Cellular Endocrinology, 2018, vol. 178, no. 1–2, pp. 117–131. https://doi.org/10.1016/S0303-7207(01)00433-6Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines / C. Gasnier [et al.] // Toxicology. – 2009. – Vol. 262, № 3. – P. 184–191. https://doi.org/10.1016/j.tox.2009.06.006Gasnier C., Dumont C., Benachour N., Clair E., Chagnon M.-C., Séralini G.-E. Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines. Toxicology, 2009, vol. 262, no. 3, pp. 184–191. https://doi.org/10.1016/j.tox.2009.06.006Kwiatkowska, M. The effect of metabolites and impurities of glyphosate on human erythrocytes (in vitro) / M. Kwiatkowska, B. Huras, B. Bukowska // Pestic. Biochem. Physiol. – 2014. – Vol. 109. – Р. 34–43. https://doi.org/10.1016/j.pestbp.2014.01.003Kwiatkowska M., Huras B., Bukowska B. The effect of metabolites and impurities of glyphosate on human erythrocytes (in vitro). Pesticide Biochemistry and Physiology, 2014, vol. 109, pp. 34–43. https://doi.org/10.1016/j.pestbp.2014.01.003Detection of glyphosate residues in animals and humans / M. Krüger [et al.] // J. Environ. Anal. Toxicol. – 2014. – Vol. 4, № 2. – Art. 210. https://doi.org/10.4172/2161-0525.1000210Krüger M., Schledorn P., Schrödl W., Hoppe H.-W., Lutz W., Shehata A. A. Detection of glyphosate residues in animals and humans. Journal of Environmental & Analytical Toxicology, 2014, vol. 4, no. 2, art. 210. https://doi.org/10.4172/2161-0525.1000210Samsel, А. Glyphosate supression of cytochrom P450 enzymes and amino acid biosynthesis by the gut microbiome: pathways to modern diseases / А. Samsel, S. Seneff // Entropy. – 2013. – Vol. 15, № 4. – P. 1416–1463. https://doi.org/10.3390/e15041416Samsel А., Seneff S. Glyphosate supression of cytochrom P450 enzymes and amino acid biosynthesis by the gut microbiome: pathways to modern diseases. Entropy, 2013, vol. 15, no. 4, pp. 1416–1463. https://doi.org/10.3390/e15041416Glyphosate is an inhibitor of plant cytochrome P450: functional expression of Thlaspi arvensae cytochrome P45071B1/reductase fusion protein in Escherichia coli / D. C. Lamb [et al.] // Biochem. Biophys. Res. Commun. – 1998. – Vol. 244, № 1. – P. 110–114. https://doi.org/10.1006/bbrc.1997.7988Lamb D. C., Kelly D. E., Hanley S. Z., Mehmood Z., Kelly S. L. Glyphosate is an inhibitor of plant cytochrome P450: functional expression of Thlaspi arvensae cytochrome P45071B1/reductase fusion protein in Escherichia coli. Biochemical and Biophysical Research Communications, 1998, vol. 244, no. 1, pp. 110–114. https://doi.org/10.1006/bbrc.1997.7988Cancer incidence among glyphosate-exposed pesticide applicators in the Agricultural Health Study / A. J. De Roos [et al.] // Environ. Health Perspect. – 2005. – Vol. 113, № 1. – Р. 49–54. https://doi.org/10.1289/ehp.7340De Roos A. J., Blair A., Rusiecki J. A., Hoppin J. A., Svec M., Dosemeci M., Sandler D. P., Alavanja M. C. Cancer incidence among glyphosate-exposed pesticide applicators in the Agricultural Health Study. Environmental Health Perspectives, 2005, vol. 113, no. 1, pp. 49–54. https://doi.org/10.1289/ehp.7340DNA and chromosomal damage induced in fish (Anguilla anguilla L.) by aminomethylphosphonic acid (AMPA) – the major environmental breakdown product of glyphosate / S. Guilherme [et al.] // Environ. Sci. Pollut. Res. – 2014. – Vol. 21, № 14. – P. 8730–8739. https://doi.org/10.1007/s11356-014-2803-1Guilherme S., Santos M. A., Gaivão I., Pacheco M. DNA and chromosomal damage induced in fish (Anguilla anguilla L.) by aminomethylphosphonic acid (AMPA) – the major environmental breakdown product of glyphosate. Environmental Science and Pollution Research, 2014, vol. 21, no. 14, pp. 8730–8739. https://doi.org/10.1007/s11356-014-2803-1Acute and chronic toxicity of glyphosate compounds to glochidia and juveniles of Lampsilis siliquoidea (unionidae) / R. B. Bringolf [et al.] // Environ. Toxico. Chem. – 2007. – Vol. 26, № 10. – P. 2094–2100. https://doi.org/10.1897/06-519R1.1Bringolf R. B., Cope W. G., Mosher S., Barnhart M. C., Shea D. Acute and chronic toxicity of glyphosate compounds to glochidia and juveniles of Lampsilis siliquoidea (unionidae). Environmental Toxicology and Chemistry, 2007, vol. 26, no. 10, pp. 2094–2100. https://doi.org/10.1897/06-519R1.1Relyea, R. A. The lethal impact of Roundup on aquatic and terrestrial amphibians / R. A. Relyea // Ecol. Appl. – 2005. – Vol. 15, № 4. – P. 1118–1124. https://doi.org/10.1890/04-1291Relyea R. A. The lethal impact of Roundup on aquatic and terrestrial amphibians. Ecological Applications, 2005, vol. 15, no. 4, pp. 1118–1124. https://doi.org/10.1890/04-1291Glyphosate-based herbicides reduce the activity and reproduction of earthworms and lead to increased soil nutrient concentrations / M. Gaupp-Berghausen [et al.] // Sci. Rep. – 2015. – Vol. 5. – Art. 12886. https://doi.org/10.1038/srep12886Gaupp-Berghausen M., Hofer M., Rewald B., Zaller J. G. Glyphosate-based herbicides reduce the activity and reproduction of earthworms and lead to increased soil nutrient concentrations. Scientific Reports, 2015, vol. 5, art. 12886. https://doi.org/10.1038/srep12886Metabolism and degradation of glyphosate in soil and water / M. L. Ruepple [et al.] // J. Agric. Food. Chem. – 1977. – Vol. 25, № 3. – P. 517–528. https://doi.org/10.1021/jf60211a018Ruepple M. L., Brightwell B. B., Schaefer J., Marvel J. T. Metabolism and degradation of glyphosate in soil and water. Journal of Agricultural and Food Chemistry, 1977, vol. 25, no. 3, pp. 517–528. https://doi.org/10.1021/jf60211a018Balthazor, T. M. Glyphosat-degrading microorganisms from industrial activated sludge / T. M. Balthazor, L. E. Hallas // Appl. Environ. Microbiol. – 1986. – Vol. 51, № 2. – P. 432–434. https://doi.org/10.1128/aem.51.2.432-434.1986Balthazor T. M., Hallas L. E. Glyphosat-degrading microorganisms from industrial activated sludge. Applied and Environmental Microbiology, 1986, vol. 51, no. 2, pp. 432–434. https://doi.org/10.1128/aem.51.2.432-434.1986Metabolism of glyphosate in Pseudomonas sp. strain Lbr / G. S. Jacob [et al.] // Appl. Environ. Microbiol. – 1988. – Vol. 54, № 12. – P. 2953–2958. https://doi.org/10.1128/aem.54.12.2953-2958.1988Jacob G. S., Garbow J. R., Hallas L. E., Kimack N. M., Kishore G. M., Schaefer J. Metabolism of glyphosate in Pseudomonas sp. strain Lbr. Applied and Environmental Microbiology, 1988, vol. 54, no. 12, pp. 2953–2958. https://doi.org/10.1128/aem.54.12.2953-2958.1988Скрининг зональных изолятов Pseudomonas sp. по устойчивости к глифосату и его утилизации как источника углерода и фосфора / Н. А. Михайловская [и др.] // Почвоведение и агрохимия. – 2021. – № 2 (67). – С. 35–48.Mikhailouskaya N. A., Barashenko T. B., Pogirnitskaya T. V., Dyusova S. V. Screening of zonal isolates Pseudomonas sp. tolerance to glyphosate and its utilization as a source of carbon and phosphorus. Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2021, no. 2 (67), pp. 35–48 (in Russian).Скрининг способности калиймобилизующих ризобактерий метаболизировать гербицид глифосат / Н. А. Михайловская [и др.] // Почвоведение и агрохимия. – 2022. – № 1 (68). − С. 200–212. https://doi.org/10.47612/0130-8475-2022-1(68)-200-212Mikhailouskaya N. A., Barashenko T. B., Pogirnitskaya T. V., Dyusova S. V. Screening the capability of potassium mobilizing rhizobacteria to metabolise herbicide glyphosate. Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2022, no. 1 (68), pp. 200–212 (in Russian). https://doi.org/10.47612/0130-8475-2022-1(68)-200-212Скрининг азотфиксирующих бактерий по способности метаболизировать гербицид глифосат как источник фосфора / Н. А. Михайловская [и др.] // Почвоведение и агрохимия. – 2022. – № 2 (69). – С. 110–120. https://doi.org/10.47612/0130-8475-2022-2(69)-110-120Mikhailouskaya N. A., Barashenko T. B., Pogirnitskaya T. V., Dyusova S. V. Screening the capability of nitrogen fixing bacteria to metabolise herbicide glyphosate as a sourse of phosphorus. Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2022, no. 2 (69), pp. 110–120 (in Russian). https://doi.org/10.47612/0130-8475-2022-2(69)-110-120Скрининг фосфатрастворяющих ризобактерий Pseudomonas spp. по активности культурального роста в зависимости от содержания глифосата в жидкой среде Дворкина-Фостера / Н. А. Михайловская [и др.] // Почвоведение и агрохимия. – 2023. – № 1 (70). – С. 136–148.Mikhailouskaya N. A., Barashenko T. B., Pogirnitskaya T. V., Dyusova S. V. Screening of phosporus solubilizing rhizospfere bacteria Pseudomonas spp. growth activity cultural in dependence on glyphosate content in liquid Dworkin-Foster medium. Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2023, no. 1 (70), pp. 136–148 (in Russian).Quinn, J. P. Carbon-phosphorus bond cleavage by Gram-positive and Gram-negative soil bacteria / J. P. Quinn, J. M. Peden, R. E. Dick // Appl. Microbiol. Biotechnol. – 1989. – Vol. 31, № 3. – Р. 283–287. https://doi.org/10.1007/BF00258410Quinn J. P., Peden J. M., Dick R. E. Carbon-phosphorus bond cleavage by Gram-positive and Gram-negative soil bacteria. Applied Microbiology and Biotechnology, 1989, vol. 31, no. 3, pp. 283–287. https://doi.org/10.1007/BF00258410Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil / J. Fan [et al.] // J. Gen. Appl. Microbiol. – 2012. – Vol. 58, N 4. – Р. 263–271. https://doi.org/10.2323/jgam.58.263Fan J., Yang G., Zhao H., Shi G., Geng Y., Hou T., Tao K. Isolation, identification and characterization of a glyphosatedegrading bacterium, Bacillus cereus CB4, from soil. The Journal of General and Applied Microbiology, 2012, vol. 58, no. 4, pp. 263–271. https://doi.org/10.2323/jgam.58.263Kishore, G. M. Degradation of glyphosate by Pseudomonas sp. PG2982 via a sarcosine intermediate / G. M. Kishore, G. S. Jacob // J. Biol. Chem. – 1987. – Vol. 262, № 25. – P. 12164–12168. https://doi.org/10.1016/s0021-9258(18)45331-8Kishore G. M., Jacob G. S. Degradation of glyphosate by Pseudomonas sp. PG2982 via a sarcosine intermediate. Journal of Biological Chemistry, 1987, vol. 262, no. 25, pp. 12164–12168. https://doi.org/10.1016/s0021-9258(18)45331-8Dick, R. E. Control of glyphosate uptake and metabolism in Pseudomonas sp. 4ASW / R. E. Dick, J. P. Quinn // FEMS Microbiol. Lett. – 1995. – Vol. 134, № 2–3. – P. 177–182. https://doi.org/10.1016/0378-1097(95)00400-9Dick R. E., Quinn J. P. Control of glyphosate uptake and metabolism in Pseudomonas sp. 4ASW. FEMS Microbiology Letters, 1995, vol. 134, no. 2–3, pp. 177–182. https://doi.org/10.1016/0378-1097(95)00400-9Degradation of the herbicide glyphosate by members of the family Rhizobiaceae / C. M. Liu [et al.] // Appl. Environ. Microbiol. – 1991. – Vol. 57, № 6. – P. 1799–1800. https://doi.org/10.1128/aem.57.6.1799-1804.1991Liu C.-M., McLean P. A., Sookdeo C. C., Cannon F. C. Degradation of the herbicide glyphosate by members of the family Rhizobiaceae. Applied and Environmental Microbiology, 1991, vol. 57, no. 6, pp. 1799–1804. https://doi.org/10.1128/aem.57.6.1799-1804.1991Li, H. Degradation and isotope source tracking of glyphosate and aminomethylphosphonic acid / H. Li, S. R. Joshi, D. P. Jaisi // J. Agric. Food. Chem. – 2016. – Vol. 64, № 3. – P. 529–538. https://doi.org/10.1021/acs.jafc.5b04838Li H., Joshi S. R., Jaisi D. P. Degradation and isotope source tracking of glyphosate and aminomethylphosphonic acid. Journal of Agricultural and Food Chemistry, 2016, vol. 64, no. 3, pp. 529–538. https://doi.org/10.1021/acs.jafc.5b04838Kamat, S. S. The enzymatic conversion of phosphonates to phosphate by bacteria / S. S. Kamat, F. M. Raushel // Curr. Opin. Chem. Biol. – 2013. – Vol. 17, № 4. – P. 589–596. https://doi.org/10.1016/j.cbpa.2013.06.006Kamat S. S., Raushel F. M. The enzymatic conversion of phosphonates to phosphate by bacteria. Current Opinion in Chemical Biology, 2013, vol. 17, no. 4, pp. 589–596. https://doi.org/10.1016/j.cbpa.2013.06.006Hove-Jensen, B. Utilization of glyphosate as phosphate source: biochemistry and genetics of bacterial carbon-phosphorus lyase / B. Hove-Jensen, D. L. Zechel, B. Jochimsen // Microbiol. Mol. Biol. Rev. – 2014. – Vol. 78, № 1. – P. 176–197. https://doi.org/10.1128/MMBR.00040-13Hove-Jensen B., Zechel D. L., Jochimsen B. Utilization of glyphosate as phosphate source: biochemistry and genetics of bacterial carbon-phosphorus lyase. Microbiology and Molecular Biology Reviews, 2014, vol. 78, no. 1, pp. 176–197. https://doi.org/10.1128/MMBR.00040-13White, A. K. Two C–P lyase operons in Pseudomonas stutzeri and their roles in the oxidation of phosphonates, phosphate, and hypophosphite / A. K. White, W. W. Metcalf // J. Bacteriol. – 2004. – Vol. 186, № 14. – P. 4730–4739. https://doi.org/10.1128/JB.186.14.4730-4739.2004White A. K., Metcalf W. W. Two C–P lyase operons in Pseudomonas stutzeri and their roles in the oxidation of phosphonates, phosphate, and hypophosphite. Journal of Bacteriology, 2004, vol. 186, no. 14, pp. 4730–4739. https://doi.org/10.1128/JB.186.14.4730-4739.2004Активность фосфатмобилизации у ризобактерий / Н. А. Михайловская [и др.] // Почвоведение и агрохимия. – 2007. – № 1 (38). – С. 225–231.Mikhailovskaya N. A., Mikanova O., Barashenko T. B., Barashenko T. V. Phosphate mobilization activity in rhizobacteria. Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2007, no. 1 (38), pp. 225–231 (in Russian).Влияние фосфатмобилизующих бактерий на ростовые процессы, урожайность и фитопатологическое состояние посевов зерновых культур на дерново-подзолистых супесчаных почвах / Н. А. Михайловская [и др.] // Почвоведение и агрохимия. – 2012. – № 1 (48). – С. 136–149.Mikhailouskaya N. A., Bogdevitch I. M., Mikanova O., Tarasiuk E. G., Barashenko T. B., Duysova S. V., Pogirnitskaya T. V. Influence of phosphorus-mobilizing bacteria on growth, yield and phytopathology state of cereal crop growings on luvisol loamy sand soils. Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2012, no. 1 (48), pp. 136–149 (in Russian).Михайловская, Н. А. Азоспириллы и их влияние на злаковые культуры (обзор литературы) / Н. А. Михайловская // Почвоведение и агрохимия. − 2015. − № 2 (55). − С. 167−181.Mikhailouskaya N. А. Azospirillum spp. and their influence on grain crop (review). Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2015, no. 2 (55), pp. 167–181 (in Russian).Михайловская, Н. А. Количественная оценка активности калиймобилизующих бактерий и их эффективность на посевах озимой ржи / Н. А. Михайловская // Вес. Нац. акад. навук Беларусi. Сер. aграр. навук. – 2006. – № 3. – С. 41–46.Mikhailovskaya N. A. Quantitative estimation of the K-mobilizing bacteria activity and application for winter rye growing. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya agrarnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Agrarian series, 2006, no. 3, pp. 41–46 (in Russian).Михайловская, Н. А. Антагонистическая активность ризобактерий A. brasilense и B. circulans по отношению к фитопатогенным микромицетам рр. Fusarium и Alternaria / Н. А. Михайловская, Т. Б. Барашенко // Почвоведение и агрохимия. – 2019. – № 1 (62). – С. 234–244.Mikhailovskaya N. A., Barashenko T. B. Antagonistic activity of rhizobacteria A. brasilense и B. circulans in respect of pathogenic fungi Fusarium и Alternaria spp. Pochvovedenie i agrokhimiya [Soil Science and Agrochemistry], 2019, no. 1 (62), pp. 234–244 (in Russian).

The authors declare that there are no conflicts of interest present.