vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2023-61-2-133-140vestiag-700Research ArticleЗЕМЛЯРОБСТВА І РАСЛІНАВОДСТВAAGRICULTURE AND PLANT CULTIVATIONСимптомы проявления Pepino mosaic virus на тепличных томатах и тест-растенияхSymptoms of Pepino mosaic virus in greenhouse tomatoes and reactions of test plants on infectionВабищевичВ. В.VabishchevichV. V.

Вабищевич Виктория Викторовна – кандидат биологических наук, ведущий специалист

(ул. Некрасова, 114, 220068, Минск

Viktoria V. Vabishchevich – Ph. D. (Biology), Leading Researcher

114, Nekrasova Str., 220068, Minsk

vv@valdisagro.byhttps://orcid.org/0000-0003-4898-1605ВолчкевичИ. Г.VolchkevichI. G.

Волчкевич Ирина Георгиевна – кандидат сельскохозяйственных наук, заведующий лабораторией защиты овощных культур и картофеля

ул. Мира, 2, 223011, аг. Прилуки, Минский район, Минская область

Irina G. Volchkevich – Ph. D. (Agriculture), Head of the Laboratory for the Protection of Vegetable Crops and Potatoes

2, Mira Str., 223011, agro-town Priluki, Minsk District, Minsk Region

onionprotect@yandex.byКонопацкаяМ. В.KanapatskayaM. V.

Конопацкая Марина Владимировна – старший научный сотрудник лаборатории защиты овощных культур и картофеля

ул. Мира, 2, 223011, аг. Прилуки, Минский район, Минская область

Marina V. Kanapatskaya – Senior Researcher of the Laboratory for the Protection of Vegetable Crops and Potatoes

2, Mira Str., 223011, agro-town Priluki, Minsk District, Minsk Region

bmarinaw@yandex.byООО «ВалдисАгро»LLC “ValdisAgro”Институт защиты растений, Национальная академия наук БеларусиInstitute of Plant Protection, National Academy of Sciences of Belarus202305052023612133140Copyright © Вабищевич В.В., Волчкевич И.Г., Конопацкая М.В., 20232023Вабищевич В.В., Волчкевич И.Г., Конопацкая М.В.Vabishchevich V.V., Volchkevich I.G., Kanapatskaya M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/700

Pepino mosaic virus является опасным возбудителем, поражающим растения томата в условиях защищенного грунта. Исследования по выявлению вируса на территории республики, проведенные в период 2019–2020 гг. при маршрутных обследованиях тепличных посадок различных гибридов культуры, показали наличие возбудителя в 54 образцах из 194 проанализированных с применением иммуноферментного анализа. Установлено, что Pepino mosaic virus встречается как в моноинфекции, так и в комплексе с такими вирусами, как Cucumber mosaic virus, Tobacco mosaic virus, Tomato mosaic virus и Potato virus X. Среди типичных симптомов проявления вируса при инфицировании растений томата отмечали межжилковый хлороз, мозаичность и деформацию листовой пластинки, а также неравномерное созревание плодов. В лабораторных условиях проведена оценка реакции 10 видов тест-растений на инокуляцию Pepino mosaic virus. Наибольшую восприимчивость проявили Nicotiana rustica L. и Datura stramonium L., у которых отмечали яркую внешнюю реакцию на заражение патогеном и высокое содержание вирусных частиц спустя 4 недели после заражения: 0,952–1,013 единицы оптической плотности. Представленные результаты могут быть использованы для диагностики Pepino mosaic virus при проведении мониторинга посадок томата закрытого грунта.

This study was carried out to detect the Pepino mosaic virus in various tomato hybrids grown in greenhouses. Total of 194 plant sample were collected from the greenhouse during 2019–2020 years. As a results of DAS-ELISA was found 54 of samples with Pepino mosaic virus, which was identified both in monoinfection and in the complex with other viruses: Cucumber mosaic virus, Tobacco mosaic virus, Tomato mosaic virus and Potato virus X. The possible symptoms of Pepino mosaic virus during the growing season of tomatoes include interveinal chlorosis, deformations, mosaic and yellow spots on leaves and also blotchy ripening fruits. The reaction of 10 plant species to the inoculation of Pepino mosaic virus was established. The results showed the greatest susceptibility of Nicotiana rustica L. and Datura stramonium L., where the maximum concentration of viral particles was detected 4 weeks after infection (OD: 0.952–1.013). The results presented in the article can be used to diagnose Pepino mosaic virus during the monitoring of greenhouse tomato plantations.

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The authors declare that there are no conflicts of interest present.