vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2022-60-1-91-104vestiag-619Research ArticleЖЫВЁЛАГАДОЎЛЯ І ВЕТЭРЫНАРНАЯ МЕДЫЦЫНАANIMAL HUSBANDRY AND VETERINARY MEDICINEВлияние адаптогена аскорбата лития на микробиоту рубца овец-ярокEffect of adaptogen lithium ascorbate on the microbiota of rumen in young ewesОстренкоК. С.OstrenkoK. S.

Остренко Константин Сергеевич – доктор биологических наук, зав. лабораторией микробиологии и иммунобиотехнологии.

П. Институт, Боровск, 290013, Калужская обл.

Konstantin S. Ostrenko - D. Sc. (Biological).

Township Institute, Borovsk 290013, Kaluga Region

ostrenkoks@gmail.comКолосковаЕ. М.KoloskovaE. M.

Колоскова Елена Михайловна – кандидат биологических наук, старший научный сотрудник лаборатории пищеварения и межуточного обмена.

П. Институт, Боровск, 290013, Калужская обл.

Elena M. Koloskova - Ph.D. (Biological).

Township Institute, Borovsk 290013, Kaluga Region

heleko3@yandex.ruЕзерскийВ. А.EzerskijV. V.

Езерский Вадим Аркадьевич – младший научный сотрудник лаборатории микробиологии и иммунобиотехнологии.

П. Институт, Боровск, 290013, Калужская обл.

Vadim A. Ezerskij - Research assistant scientist.

Township Institute, Borovsk 290013, Kaluga Region

ez.vadim@yandex.ruОвчароваА. Н.OvcharovaA. N.

Овчарова Анастасия Никитовна – кандидат биологических наук, старший научный сотрудник лаборатории микробиологии и иммунобиотехнологии.

П. Институт, Боровск, 290013, Калужская обл.

Anastasiya N. Ovcharova - Ph.D. (Biological).

Township Institute, Borovsk 290013, Kaluga Region

naka7@yandex.ruБеловаН. В.BelovaN. V.

Белова Надежда Викторовна – младший научный сотрудник лаборатории микробиологии и иммунобиотехнологии.

П. Институт, Боровск, 290013, Калужская обл.

Nadezhda V. Belova - Research assistant scientist.

Township Institute, Borovsk 290013, Kaluga Region

navikbel@mail.ruИльинаЛ. А.Il’inaL. A.

Ильина Лариса Александровна – кандидат биологических наук, начальник молекулярно-генетической лаборатории.

Загребский б-р, 19, корп. 1, 192284, Санкт-Петербург

Larisa A. Il’ina - Ph.D. (Biological). Head of the molecular genetic laboratory.

19, korp. 1, Zagrebskiibulv., St. Petersburg, 192284

ilina@biotrof.ruЙылдырымЕ. А.JyldyrymE. A.

Йылдырым Елена Александровна – доктор биологических наук, главный биотехнолог.

Загребский б-р, 19, корп. 1, 192284, Санкт-Петербург

Elena A. Jyldyrym - D. Sc. (Biological)

19, korp. 1, Zagrebskiibulv., St. Petersburg, 192284

ideniz@biotrof.ruЛаптевГ. Ю.LaptevG. Yu.

Лаптев Георгий Юрьевич – доктор биологических наук, директор.

Загребский б-р, 19, корп. 1, 192284, Санкт-Петербург

Georgij Yu. Laptev - D. Sc. (Biological), director.

19, korp. 1, Zagrebskiibulv., St. Petersburg, 192284

Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных, филиал ФГБНУ Федеральный исследовательский центр животноводства – ВИЖ имени академика Л.К. ЭрнстаAll-Russian research Institute of Physiology, Biochemistry and Nutrition of animals - branch of the Federal Science Center for Animal Husbandry named after Academy Member L. K. ErnstБиотроф, ОООBiotrof, LLC20220202202260191104Copyright © Остренко К.С., Колоскова Е.М., Езерский В.А., Овчарова А.Н., Белова Н.В., Ильина Л.А., Йылдырым Е.А., Лаптев Г.Ю., 20222022Остренко К.С., Колоскова Е.М., Езерский В.А., Овчарова А.Н., Белова Н.В., Ильина Л.А., Йылдырым Е.А., Лаптев Г.Ю.Ostrenko K.S., Koloskova E.M., Ezerskij V.V., Ovcharova A.N., Belova N.V., Il’ina L.A., Jyldyrym E.A., Laptev G.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/619

Здоровье и продуктивность жвачных животных напрямую зависят от рубцового и кишечного пищеварения. Нормальная микрофлора защищает организм от патогенных микробов, стимулирует иммунную систему, участвует в метаболических реакциях и играет важную роль в энергетическом обмене. Нарушение видового состава микрофлоры рубца под влиянием различных факторов приводит к изменению усвояемости продуктов пищеварения, изменениям ферментативных процессов и развитию прочих патологических процессов. Биологически активные вещества адаптогенной природы могут проявлять иммуномодулирующую и антимикробную активность. Адаптоген аскорбат лития обладает стресс-протекторными, нейротрофическими, нейропротекторными, антиоксидантными, иммуномодулирующими свойствами и в качестве кормовой добавки влияет на микробиоту рубца. В настоящем исследовании при использовании NGS-секвенирования впервые изучено влияние адаптогена аскорбата лития на бактериальное сообщество рубца овец-ярок романовской породы. На фоне длительного содержания овец на основном рационе в условиях вивария ВНИИФБиП нормофлора рубца не подвергалась негативным изменениям. Введение органической соли лития в состав основного рациона в дозировке 10 мг/кг живой массы способствовало усилению процессов ферментации в рубце, достоверному росту целлюлозолитической активности, снижению суммарной доли патогенных и доли некоторых условно-патогенных микроорганизмов в рубцовом содержимом. Полученные данные свидетельствуют о благотворном влиянии введения аскорбата лития в качестве добавки к основному рациону на состав микробиоты рубца овец.

Health and productivity of ruminants directly depend on the state of rumen and digestion in intestinal tract. Normal microflora protects the body from pathogenic microbes, stimulates the immune system, participates in metabolic reactions and plays an important role in energy metabolism. Disturbance of species composition of microflora in rumen under effect of various factors leads to a state of dysbiosis, disorders of products digestibility, changes in enzymatic processes and other disorders. Biologically active substances of an adaptogenic nature can show immunomodulatory and antimicrobial activity. Adaptogen lithium ascorbate has stress-protective, neurotrophic, neuroprotective, antioxidant, immunomodulatory properties. In this paper, using molecular genetic methods, the effect of lithium ascorbate on composition of rumen microbiota of sheep was studied for the first time.  Composition of microbiota was determined by NGS sequencing method. In total, 37 phylums, 76 classes, 98 orders, 225 families and 894 species of microorganisms were identified. It has been determined that introduction of lithium ascorbate at a dose of 10 mg/kg of body weight into the main diet of sheep contributed to increase in proportion of cellulolytic bacteria from 73.6±1.6% to 75.4±0.9% and over 40% decrease in total number of infusoria in rumen content. Cellulolytic activity of rumen fluid in sheep of the experimental group increased by 38% compared to the animals of the control group. The number of pathogenic bacteria decreased from 2.7% in sheep of the control group to 1.6% in animals of the experimental group, while the content of beneficial bacilli increased from 0.3% to 0.5%, respectively. Content of undesirable and conditionally pathogenic microflora decreased in the sheep of experimental group. The obtained data confirm positive effect of adaptogen lithium ascorbate, introduced into diet at a dosage of 10 mg/kg of body weight, on qualitative and quantitative composition of microbiota in sheep rumen. Therefore, lithium ascorbate, a broad-spectrum adaptogen, can be recommended as a feed additive to the main diet of sheep to improve rumen digestion and increase productivity.

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The authors declare that there are no conflicts of interest present.