vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2022-60-2-69-78vestiag-617Research ArticleЖЫВЁЛАГАДОЎЛЯ І ВЕТЭРЫНАРНАЯ МЕДЫЦЫНАANIMAL HUSBANDRY AND VETERINARY MEDICINEГенетическая специфичность белоголовой украинской породы по гену BоLA-DRB3Genetic specificity of the white-headed ukrainian breed according to the BоLA-DRB3 genehttps://orcid.org/0000-0003-4708-6692СупровичТ. М.SuprovychT. M.

Супрович Татьяна Михайловна – доктор сельскохозяйственных наук, профессор.

Ул. Шевченка, 13, 32400 Каменец-Подольский, Хмельницкая область

Tetiana M. Suprovych - D.Sc. (Agricultural), Professor.

13, Shevchenko, Kamianets-Podilskyi 32400

suprovycht@gmail.comhttps://orcid.org/0000-0003-0888-662XБирюковаО. Д.BiriukovaO. D.

Бирюкова Ольга Дмитриевна – кандидат сельскохозяйственных наук, старший научный сотрудник.

Ул. Погребняка, 1, 08321 с. Чубинское, Бориспольский р-н, Киевская область

Olga D. Biriukova - Ph.D. (Agricultural).

1, Pogrebnyaka, Chubynske 08321, Boryspil district, Kiev region

irgt.spetsrada@ukr.nethttps://orcid.org/0000-0001-6614-8823СупровичН. П.SuprovychM. P.

Супрович Николай Петрович – кандидат технических наук, доцент.

Ул. Шевченка, 13, 32400 Каменец-Подольский, Хмельницкая область

Mykola P. Suprovych - Ph.D. (Engineering), Assistant Professor.

13, Shevchenko, 32400, Kamianets-Podilskyi

kokas2008@ukr.nethttps://orcid.org/0000-0002-7129-5518ЧепурнаяВ. А.ChepurnaV. A.

Чепурная Валентина Анатольевна – аспирант, Подольский государственный университет.

Ул. Шевченка, 13, 32400 Каменец-Подольский, Хмельниц кая область

Valentyna A. Chepurna - Postgraduate.

13, Shevchenko, 32400, Kamianets-Podilskyi

valentinachepurna70@gmail.comhttps://orcid.org/0000-0002-5693-916XКарчевскаяТ. Н.KarchevskaT. M.

Карчевская Татьяна Николаевна – кандидат сельскохозяйственных наук, доцент.

Ул. Шевченка, 13, 32400 г. Каменец-Подольский, Хмельницкая область

Tetiana M. Karchevska - Ph.D. (Veterinary), Assistant Professor.

13, Shevchenko, 32400, Kamianets-Podilskyi

ktmkp2015@gmail.comhttps://orcid.org/0000-0002-9669-4144КолодийВ. А.KolodiiV. A.

Колодий Валентина Анатольевна – кандидат биологических наук, ассистент.

Ул. Огиенка, 61, 32400 Каменец-Подольский, Хмельницкая область

Valentyna A. Kolodii - Ph.D. (Biological).

61, Ohienko, Kamianets-Podilskyi, 32400

kolodiyva@ukr.netЛеснякЮ. И.LesniakYu. I.

Лесняк Юрий Иванович – кандидат сельскохозяйственных наук, старший научный сотрудник.

Ул. Погребняка, 1, 08321 с. Чубинское, Бориспольский р-н, Киевская область

Yuriy I. Lesniak - Ph.D. (Agricultural).

1, Pogrebnyaka, Chubynske 08321, Boryspil district, Kiev region

bioclar@ukr.netПодольский аграрный университетPodillia State UniversityИнститут разведения и генетики животных имени М. В. Зубца, НААНInstitute of Animal Breeding and Genetic nd.а.M.V. Zubets, NAAS of UkraineКаменец-Подольский государственный университет им. И. ОгиенкоKamianets-Podilskyi Ivan Ohiienko National University2022020220226016978Copyright © Супрович Т.М., Бирюкова О.Д., Супрович Н.П., Чепурная В.А., Карчевская Т.Н., Колодий В.А., Лесняк Ю.И., 20222022Супрович Т.М., Бирюкова О.Д., Супрович Н.П., Чепурная В.А., Карчевская Т.Н., Колодий В.А., Лесняк Ю.И.Suprovych T.M., Biriukova O.D., Suprovych M.P., Chepurna V.A., Karchevska T.M., Kolodii V.A., Lesniak Y.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/617

Изучение генетической специфичности локальных пород – одно из перспективных направлений в контексте сохранения биоразнообразия пород крупного рогатого скота в мире. Цель исследования – определение и анализ аллельного полиморфизма гена BoLA-DRB3 белоголовой украинской породы. Изучение проведено на образцах крови 49 животных (11 быков и 38 коров) этой породы. Аллельный спектр гена BoLA-DRB3 определяли на основе двухступенчатой ПЦР (праймеры HLO-30, HLO-31 и HLO-32). Рестрикцию проводили эндонуклеазами RsaI, HaeIII, BstYI. Фрагменты рестрикции разделяли электрофорезом в 9–12%-ном полиакриламидном геле. В результате исследований обнаружено 29 аллелей. Семь аллелей (*03, *11, *13, *15, *22, *23 и *24) определялись с частотой более 5 %, что составляло 65,3 % аллельного фонда породы. Наиболее распространенным с частотой 15,3 % выявлялся аллель *24. В экспериментальной выборке идентифицировано 37 генотипов. Преобладающий вариант – *11/*24 (16,2 %). Выявлен небольшой избыток гетерозигот (FIS = -0,035). Характерной особенностью белоголового украинского скота является существенная дифференциация (или специфичность) по гену BoLA-DRB3 (индекс фиксации Райта He = 0,959, индекс Шеннона-Винера H` = 2,93), что подтверждает тезис о важной роли местных пород в сохранении биоразнообразия генетических ресурсов крупного рогатого скота.

The study of the genetic specificity of local breeds is a promising direction in the context of preserving the biodiversity of cattle breeds in the world. The purpose of research was to analyze the allelic polymorphism of the BoLA-DRB3 gene of the White-Headed Ukrainian breed. The study was carried out with blood samples from 49 animals (11 bulls and 38 cows) of this breed. The allelic spectrum of the BoLA-DRB3 gene was detected based on two-step PCR (primers HLO-30, HLO-31 and HLO-32). Restriction was performed with endonucleases RsaI, HaeIII, BstYI. Restriction fragments were separated by electrophoresis in 9-12 % polyacrylamide gel. According to the test results, 29 alleles were detected. Seven alleles (*03, *11, *13, *15, *22, *23 and *24) were determined with a frequency of over 5 %, that was 65.3 % of the allele pool of the breed. The most common was allele *24 with a frequency of 15.3%. In the experimental sample, 37 genotypes were identified. The predominant variant was *11/*24 (16.2%). A slight excess of heterozygotes was detected (Fis = – 0.035). White-Headed Ukrainian breed is characterized by a significant level of differentiation (or specificity) according to the BoLA-DRB3 gene (Wright fixation index He = 0.959, Shannon-Wiener index H` = 2.93), that confirms the thesis of the important role of local breeds in preserving the biodiversity of genetic resources of cattle.

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The authors declare that there are no conflicts of interest present.