vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2021-59-3-361-365vestiag-584Research ArticleЖЫВЁЛАГАДОЎЛЯ І ВЕТЭРЫНАРНАЯ МЕДЫЦЫНАANIMAL HUSBANDRY AND VETERINARY MEDICINEГенотипирование «украинских» водных буйволов по генам β-CN (A2 – молоко), CSN3 и βLGGenotyping of “Ukrainian” water buffaloes according β-CN (A2-milk), CSN3 and βLG geneshttps://orcid.org/0000-0001-5982-6542МохначеваН. Б.MokhnachovaN. B.

Мохначева Наталия Борисовна – кандидат сельскохозяйственных наук, старший научный сотрудник

ул. Погребняка, 1, с. Чубинское, Бориспольский р-н Киевская область, 08321

Nataliia B. Mokhnachova - Ph. D. (Agricultural)

1, Pogrebnyaka Str., 08321 Chubinskoe, Boryspil district, Kiev region

nataliia.mokhnachova82@gmail.comИнститут разведения и генетики животных имени М.В. Зубца Национальной академии аграрных наукSenior Researcher at Institute of Animal Breeding and Genetics n.a. M.V. Zubets the National Academy of Agrarian Sciences202105082021593361365Copyright © Мохначева Н.Б., 20212021Мохначева Н.Б.Mokhnachova N.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/584

В Украине разведение буйволов является древней традиционной отраслью животноводства крымских татар и русинов Закарпатья. В основном «украинские» буйволы относятся к речному буйволу (Bubalus bubalis) и разводятся для молочного и мясного способа производства. Полиморфизм генов, ассоциированных с молочной продуктивностью, позволит вести селекцию буйволов с учетом «желанных» генотипов в отношении хозяйственно полезных признаков. В статье исследованы аллельные полиморфизмы генов бета-казеина (β-CN), каппа-казеина (CSN3) и беталактоглобулина (β-LG) в популяции водных буйволов, которые разводятся в Украине, с помощью ПЦР с последующим рестрикционным гидролизом образующихся фрагментов (ПЦР-ПДРФ). Обсуждены результаты исследования «украинской» популяции водных буйволов, а именно: частота генотипов и аллелей в локусах генов бета-казеина, каппа-казеина и бета-лактоглобулина. Амплифицированный фрагмент β-CN длиной 121 п.н. разрезали с помощью фермента рестрикции DdeI. Характерная особенность аллельного спектра гена бета-казеина (β-CN) в изученной популяции выразилась отсутствием алеля А1. Все животные несли генотип β-CNА2А2 гена бета-казеина, соответственно аллель β-CNА2 встречался с частотой 1,0. Для гена CSN3 амплифицированный фрагмент размером 273 п.н. обрабатывали рестриктазой HinfI. Выявлено 100%-ное преобладание животных с наиболее предпочтительным гомозиготным генотипом CSN3BB. В про цессе исследования гена β-LG амплифицированный фрагмент размером 247 п.н. расщеплялся HaeIII. Установлено, что наиболее часто встречались аллель β-LGА и генотип β-LGАА гена бета-лактоглобулина (0,96 и 0,92 соответственно). Гетерозигоный β-LGАВ-генотип присутствует у 8 % буйволов. Результаты исследований представляют интерес в области молекулярно-генетического анализа генома буйволов, которые являются источником специфических свойств. Полученные данные могут быть полезными для сохранения и увеличения генетического разнообразия «украинской» популяции водных буйволов, а также с целью получения от буйволов ценной продукции.

Buffalo breeding in Ukraine is an ancient traditional branch of animal husbandry of the Crimean Tatars and Rusyns of Transcarpathia. Basically, "Ukrainian" buffaloes belong to the river buffalo (Bubalus bubalis) and are bred for dairy and meat production. Polymorphism of genes associated with dairy productivity will allow breeding buffaloes taking into account the “desired” genotypes in relation to economically useful traits. The paper dwells on studying allelic polymorphisms of beta-casein (β-CN), kappa-casein (CSN3) and beta-lactoglobulin (β-LG) genes in population of water buffaloes bred in Ukraine using PCR followed by restriction hydrolysis of the formed fragments (PCR-RFLP). Results of study of the “Ukrainian” population of water buffaloes are discussed, namely: the frequency of genotypes and alleles at the loci of beta-casein, kappa-casein and beta-lactoglobulin genes. Amplified fragment β-CN with the length of 121 bp was digested with DdeI restriction enzyme. A feature of the allelic spectrum of the beta-casein gene (β-CN) in the studied population was absence of A1 allele. All animals carried the β-CNA2A2 genotype of beta-casein gene, respectively, β-CNA2 allele frequency was 1.0. For the CSN3 gene, an amplified fragment in 273 bp was digested with HinfI restriction enzyme. A 100% predominance of animals with the most preferred homozygous CSN3BB genotype was revealed. During the β-LG gene study process, an amplified fragment with a size of 247 bp was digested with HaeIII. It has been determined that the most frequent was allele βLGA and genotype βLGAA of beta-lactoglobulin gene (0.96 and 0.92, respectively). Heterozygous β-LGAB genotype is present in 8 % of buffaloes. The research results are of interest in the field of molecular genetic analysis of the buffalo genome, which are the source of specific properties. The data obtained can be useful for preserving and increasing the genetic diversity of the “Ukrainian” population of water buffaloes, as well as for obtaining valuable products from buffaloes.

крупный рогатый скотводяной буйволА2-молокогенетические маркеры продуктивностигенгенотипбета-казеинкаппа-казеинбета-лактоглобулинДНК-полиморфизмПЦР-ПДРФгенотипированиеаллельмолочная продуктивностьcattlewater buffaloA2-milkgenetic markers of productivitygenegenotypebeta-caseinkappa-caseinbetalactoglobulinDNA polymorphismPCR-RFLPgenotypingalleledairy productivityРабота выполнена в рамках программы «Генетика, сохранения и воспроизводства биоресурсов в животноводстве» на 2021–2025 годы, подпрограмма «Генетическая оценка животных референтных популяций за SNP-полиморфизмом разных локусов ДНК», ГР №0121U109254This research was performed with supported of the program 31.01.00.03. F "Genetic assessment of animal reference populations for SNP-polymorphism of different DNA loci", SR No. 0121U109254ReferencesGuzeev Yu. V. Buffalo breeding of Ukraine: past, present and possible future. Tavrіis’kii naukovii vіsnik. Seriya: Sіl’s’kogospodars’kі nauki [Taurida Scientific Herald. 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The authors declare that there are no conflicts of interest present.