vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2021-59-1-71-80vestiag-542Research ArticleЖЫВЁЛАГАДОЎЛЯ І ВЕТЭРЫНАРНАЯ МЕДЫЦЫНАANIMAL HUSBANDRY AND VETERINARY MEDICINEПолногеномный поиск ассоциаций (GWAS) с продуктивностью у овец романовской породыGenome-wide association study (GWAS) with productivity in Romanov sheep breedhttps://orcid.org/0000-0003-4536-1814КриворучкоА. Ю.KrivoruchkoA. Y.

Криворучко Александр Юрьевич – доктор биологических наук, главный научный сотрудник лаборатории геномной селекции и репродуктивной криобиологии в животноводстве

пер. Зоотехнический,15, 355017, г. Ставрополь, Ставропольский край

 

Alexander Y. Krivoruchko - D. Sc. (Biological)

15 Alley Zootechnical, Stavropol, 355017

rcvm@yandex.ruhttps://orcid.org/0000-0003-2730-2482ЯцыкО. А.YatsykO. A.

Яцык Олеся Андреевна – кандидат биологических наук, научный сотрудник лаборатории геномной селекции и репродуктивной криобиологии в животноводстве

пер. Зоотехнический,15, 355017, г. Ставрополь, Ставропольский край

 

Olesya A. Yatsyk - Ph.D. (Biological)

15 Alley Zootechnical, Stavropol, 355017

malteze@mail.ruhttps://orcid.org/0000-0002-5306-795XСаприкинаТ. Ю.SaprikinaT. Y.

Саприкина Татьяна Юрьевна – младший научный сотрудник лаборатории геномной селекции и репродуктивной криобиологии в животноводстве

пер. Зоотехнический,15, 355017, г. Ставрополь, Ставропольский край

 

Tatyana Y. Saprikina

15 Alley Zootechnical, Stavropol, 355017

saprikina.tanya@mail.ruhttps://orcid.org/0000-0001-6101-7293ПетуховаД. Д.PetukhovaD. D.

Петухова Дарья Дмитриевна – младший научный сотрудник лаборатории геномной селекции и репродуктивной криобиологии в животноводстве

пер. Зоотехнический,15, 355017, г. Ставрополь, Ставропольский край

Daria D. Petukhova

15 Alley Zootechnical, Stavropol, 355017

d1319731@yandex.ruВсероссийский научно-исследовательский институт овцеводства и козоводства – филиал Федерального государственного бюджетного научного учреждения «Северо-Кавказский федеральный научный аграрный центр»All-Russian Research Institute for Sheep and Goat Breeding - branch of the North Caucasus Federal Agricultural Research Center2021090220215917180Copyright © Криворучко А.Ю., Яцык О.А., Саприкина Т.Ю., Петухова Д.Д., 20212021Криворучко А.Ю., Яцык О.А., Саприкина Т.Ю., Петухова Д.Д.Krivoruchko A.Y., Yatsyk O.A., Saprikina T.Y., Petukhova D.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/542

Использование генетических технологий в селекции мелкого рогатого скота требует поиска новых молекулярных маркеров продуктивных качеств. Наиболее эффективным для этого является полногеномный поиск ассоциаций (GWAS) однонуклеотидных полиморфизмов (SNP) с хозяйственно ценными признаками. В статье приведены результаты исследования ассоциаций частоты однонуклеотидных полиморфизмов с ранговой оценкой по комплексу продуктивных признаков («супер-элита») у овец романовской породы при помощи ДНК-биочипов Ovine Infinium HD BeadChip 600K. Обнаружено одиннадцать SNP, имеющих достоверную связь с принадлежностью животных к группе «супер-элита». Пять замен находятся в интронах генов, шесть относятся к межгенным полиморфизмам. Наибольшая достоверность ассоциации с продуктивностью наблюдалась у замены rs410516628 (р = 3,14 ·10–9), находящейся на 3-й хромосоме. Замена rs422028000 на 2-й хромосоме отличается тем, что в группе «супер-элитных» животных она обнаруживалась в 90 % гаплотипов. Полиморфизмы rs411162754 (1-я хромосома) и rs417281100 (10‑я хромосома) в нашем исследовании оказались самыми редкими – только в группе «супер-элитных» особей и только в четверти гаплотипов. Гены, находящиеся рядом с выявленными SNP, преимущественно связаны с обменными и регуляторными процессами. Наше исследование выявило несколько новых генов-кандидатов, полиморфизм которых может быть связан с ранговой оценкой по показателям продуктивности у овец романовской породы: LTBP1, KCNH8, LMX1B, ZBTB43, MSRA, CHPF, PID1, DNER. Полученные результаты создают теоретическую базу для дальнейшего изучения генов-кандидатов, влияющих на реализацию фенотипических признаков у овец романовской породы. Выявленные полиморфизмы, ассоциированные с продуктивными качествами овец, могут быть использованы в практической селекции как молекулярно-генетические маркеры для подбора родительских пар.

Genetic technologies used in breeding of small ruminants requires searching for new molecular markers of productive traits. The most effective for this is genome-wide association study (GWAS) of single nucleotide polymorphisms (SNP) with economically valuable traits. The paper presents results of study of associations of the frequency of single nucleotide polymorphisms with a rank assessment according to complex of productive traits (super-elite) in Romanov sheep using DNA biochips Ovine Infinium HD BeadChip 600K. Eleven SNPs have been found having significant correlation with the animals belonging to the “super-elite” group. Five substitutions are located in the genes introns, six are related to intergenic polymorphisms. The highest reliability of association with productivity was observed in substitution rs410516628 (р = 3,14 · 10-9) located on the 3rd chromosome. Substitution rs422028000 on 2nd chromosome differs with the fact that in the “super-elite” group it was found in 90 % of haplotypes. Polymorphisms rs411162754 (1st chromosome) and rs417281100 (10th chromosome) in our study turned out to be the rarest – only in “super-elite” group and only in a quarter of haplotypes. The genes located near the identified SNPs are mainly associated with metabolic and regulatory processes. Our study has identified several new candidate genes with polymorphism probably associated with the ranking in terms of productivity in Romanov sheep: LTBP1, KCNH8, LMX1B, ZBTB43, MSRA, CHPF, PID1 and DNER. The results obtained create a theoretical basis for further study of candidate genes affecting implementation of phenotypic traits in Romanov sheep. The revealed polymorphisms associated with the productive traits of sheep can be used in practical breeding as molecular and genetic markers for selection of parental pairs.

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The authors declare that there are no conflicts of interest present.