vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2020-58-4-432-442vestiag-523Research ArticleЗЕМЛЯРОБСТВА І РАСЛІНАВОДСТВAAGRICULTURE AND PLANT CULTIVATIONПолучение микроклубней картофеля в жидкой питательной средеObtaining potato microtubers in a liquid nutrient mediumhttps://orcid.org/0000-0003-4609-7912ВолковД. В.VolkovD. V.

Волков Дмитрий Владимирович – магистр биологических наук, старший научный сотрудник лаборатории селекции и биотехнологии

Тимирязева 45, Алматы 050040

Dmitriy V. Volkov – M.S. (Biological)

45 Timiryazev Str., 050040, Almaty, Kazakhstan

volkovdmitriykz@gmail.comhttps://orcid.org/0000-0003-3073-4577ДауровД. Л.DaurovD. L.

Дауров Диас Ламзарович – магистр технических наук, научный сотрудник лаборатории селекции и биотехнологии

Тимирязева 45, Алматы 050040

Dias L. Daurov –M.S. (Engineering)

45 Timiryazev Str., 050040, Almaty, Kazakhstan

dias.daurov@gmail.comhttps://orcid.org/0000-0001-7949-9112ДауроваA. К.DaurovaA. K.

Айнаш Кененбайкызы Даурова – магистр сельскохозяйственных наук, научный сотрудник лаборатории селекции и биотехнологии

Тимирязева 45, Алматы 050040

Ainash K. Daurova – M.S. (Agricultural)

45 Timiryazev Str., 050040, Almaty, Kazakhstan

ainash.daurova@gmail.comhttps://orcid.org/0000-0003-1822-1437АбайЖ. С.AbayZh. S.

Абай Жандос Сайлаубекулы – бакалавр технических наук, лаборант лаборатории селекции и биотехнологии биотехнологии

Тимирязева 45, Алматы 050040

Zhandos S. Abay – B.Sc. (Engineering)

45 Timiryazev Str., 050040, Almaty, Kazakhstan

abai.zhandos15@gmail.comhttps://orcid.org/0000-0002-9007-9730ЖапарК. К.ZhaparK. K.

Жапар Куаныш Кабылулы – магистр биологических наук, младший научный сотрудник лаборатории селекции и биотехнологии

Тимирязева 45, Алматы 050040

Kuanysh K. Zhapar – M.S. (Biological)

45 Timiryazev Str., 050040, Almaty, Kazakhstan

zhapar.zk@gmail.comhttps://orcid.org/0000-0001-5243-145XЖамбакинК. Ж.ZhambakinK. Zh.

Жамбакин Кабыл Жапарович – академик НАН Республики Казахстан, доктор биологических наук, профессор, генеральный директор

Тимирязева 45, Алматы 050040

Kabl Zh. Zhambakin – Academician, D.Sc. (Biological), Professor

45 Timiryazev Str., 050040, Almaty, Kazakhstan

zhambakin@gmail.comhttps://orcid.org/0000-0002-8746-7484ШамековаМ. Х.ShamekovaM. Kh.

Шамекова Малика Хабидулаевна – ассоциированный профессор, доктор философии, заведующая лаборатории селекции и биотехнологии 

Тимирязева 45, Алматы 050040

Malika Kh. Shamekova – Associate professor, Ph.D. (Philosophy) uage: AR-SA'>(Engineering)

45 Timiryazev Str., 050040, Almaty, Kazakhstan

m.shamekova@gmail.comИнститут биологии и биотехнологии растений, Комитет науки Министерства образования и науки Республики КазахстанInstitute of Plant Biology and Biotechnology, Committee of Science of the Ministry of Education and Sciences of the Republic of Kazakhstan202003112020584432442Copyright © Волков Д.В., Дауров Д.Л., Даурова A.К., Абай Ж.С., Жапар К.К., Жамбакин К.Ж., Шамекова М.Х., 20202020Волков Д.В., Дауров Д.Л., Даурова A.К., Абай Ж.С., Жапар К.К., Жамбакин К.Ж., Шамекова М.Х.Volkov D.V., Daurov D.L., Daurova A.K., Abay Z.S., Zhapar K.K., Zhambakin K.Z., Shamekova M.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/523

Получение безвирусного семенного материала картофеля в Казахстане в основном проводится через выделение апикальных меристем с последующим получением из них пробирочных растений на агаризованной питательной среде и дальнейшем образовании из них миниклубней в закрытом грунте. Получение микроклубней из меристемных растений в производстве семенного материала картофеля in vitro не проводится, хотя этот метод является одним из успешных методов увеличения материала картофеля в условиях in vitro. Исходя из вышеуказанной проблемы нами была поставлена цель – оптимизировать жидкую питательную среду MS для получения микроклубней картофеля in vitro из меристемных растений с перспективой введения данной процедуры в процесс получения семенного материала в семеноводческих хозяйствах. В статье изложены новые данные, которые применимы на первоначальных этапах семеноводства картофеля, при производстве первого клубневого поколения здорового материала, в частности, при тиражировании растений in vitro и производстве микроклубней. Представлены результаты анализа морфометрических данных развития растений in vitro, полученных при культивировании одноузловых сегментов картофеля на десяти вариантах жидкой среды Мурасиге-Скуга с различными сочетаниями концентраций сахарозы (20 и 30 г/л), кинетина (0, 2 и 4 мг/л), гиббереловой кислоты (0, 0,5 и 1 мг/л) в стеклянных сосудах объемом 220 мл. Выявлена оптимальная жидкая питательная среда для получения развитых растений in vitro картофеля для дальнейшего производства микроклубней. Определена эффективность жидкой питательной среды для получения микроклубней картофеля при выращивании растений in vitro на средах с различными сочетаниями концентраций сахарозы (60 и 90 г/л), кинетина (0 и 2 мг/л) и 6-бензиламинопурина (0 и 5 мг/л). Результаты исследования могут стать основой для культивирования растений в биореакторе с целью массового производства свободных от вирусов микроклубней картофеля. Благодарности. Исследования проведены в рамках грантового финансирования Комитета науки Министерства образования и науки Республики Казахстан, проект AP05131947 «Использование биореактора для высокоэффективного получения безвирусного посадочного материала картофеля».

Obtaining virus-free potato seed material in Kazakhstan is mainly carried out using the method of isolation of apical meristems with the subsequent production of test-tube plants from them on an agar nutrient medium and the further formation of minitubers in greenhouse. Obtaining microtubers from meristem plants in production of potato seed material in vitro is not carried out, although this method is one of the most successful methods for increasing the potato material in vitro. Based on the above issue, we’ve set a goal to optimize the MS liquid nutrient medium for obtaining potato microtubers in vitro from meristem plants with the prospect of introducing this procedure into the process of obtaining seed material at seed farms. The paper presents new data applicable at the initial stages of potato seed production, for production of the first tuberous generation of healthy material, in particular, for replication of plants in vitro and production of microtubers. The results are presented on analysis of morphometric data of plant development in vitro obtained at cultivation of single-bud potato cuttings on ten variants of the Murashige-Skuga liquid medium with various combinations of sucrose concentrations (20 and 30 g/l), kinetin (0,2 and 4 mg/l), gibberellic acid (0, 0,5 and 1 mg/l) in 220 ml glass vessels. The perfect liquid nutrient medium for obtaining developed potato plants in vitro for further production of microtubers has been defined. Efficiency of liquid nutrient medium for obtaining potato microtubers has been determined when growing plants in vitro USING media with various combinations of concentrations of sucrose (60 and 90 g/l), kinetin (0 and 2 mg/l) and 6-benzylaminopurine (0 and 5 mg/l). The results of the study can form the basis for cultivation of plants in a bioreactor with the purpose of mass production of virus-free potato microtubers.

картофельмикроклубниминиклубникультура тканейпитательная средаоптимизациябиореакторкинетингиберелловая кислота6-бензиламинопуринсахарозаpotatomicrotubersminituberstissue culturenutrient mediumoptimizationbioreactorkinetingibberellic acid6-benzylaminopurinesucroseИсследования проведены в рамках грантового финансирования Комитета науки Министерства образования и науки Республики Казахстан, проект AP05131947 «Исполь-зование биореактора для высокоэффективного получения безвирусного посадочного материала картофеля».The research has been carried out within the framework of grant funding from the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan, project AP05131947 “Use of a bioreactor for highly efficient production of virus-free potato planting material”.ReferencesA review on potato (Solanum tuberosum L.) and its genetic diversity / B. J. Reddy [et al.] // Intern. J. of Genetics. – 2018. – vol. 10, № 2. – P. 360–364. https://doi.org/10.9735/0975-2862.10.2.360-364Reddy B. J., Mandal R., Chakroborty M., Hijam L., Dutta P. A review on potato (Solanum tuberosum L.) and its genetic diversity. International Journal of Genetics, 2018, vol. 10, no. 2, pp. 360-364. https://doi.org/doi:10.9735/09752862.10.2.360-364Muthoni, J. Alleviating potato seed tuber shortage in developing countries: potential of true potato seeds / J. Muthoni, H. Shimelis, R. Melis // Austral. J. of Crop Science. – 2013. – Vol. 7, N 12. – P. 1946–1954.Muthoni J., Shimelis H., Melis R. Alleviating potato seed tuber shortage in developing countries: Potential of true potato seeds. Australian Journal of Crop Science, 2013, vol. 7, no. 12, pp. 1946-1954.Приемы повышения продуктивности картофеля в питомниках оригинального семеноводства / Н. А. Курейчик [и др.] // Картофелеводство : сб. науч. тр. / Науч.-практ. центр НАН Беларуси по картофелеводству и плодоовощеводству. – Минск, 2013. – Вып. 21, ч. 2. – С. 72–80.Kureichik N. A., Zaboronok I. M., Sokol S. V, Zhiveto L. K. Methods for increasing the productivity of potatoes in nurseries of original seed production. Kartofelevodstvo: sbornik nauchnykh trudov [Potato growing: collection of scientific papers]. Minsk, 2013, iss. 21, pt. 2, pp. 72-80 (in Russian).Jones, E. D. A current assessment of in vitro culture and other rapid multiplication methods in North America and Europe / E. D. Jones // Amer. Potato J. – 1988. – Vol. 65, N 4. –P. 209–220. https://doi.org/10.1007/BF02854453Jones E. D. A current assessment of in vitro culture and other rapid multiplication methods in North America and Europe. American Potato Journal, 1988, vol. 65, no. 4, pp. 209-220. https://doi.org/10.1007/BF02854453Sucrose utilization during potato micro tuber growth in bioreactors / W. C. Yu [et al.] // Plant Cell Reports. – 2000. – Vol. 19, № 4. – P. 407–413. https://doi.org/10.1007/s002990050748Yu W. C., Joyce P. J., Cameron D. C., McCown B. H. Sucrose utilization during potato micro tuber growth in bioreactors. Plant Cell Reports, 2000, vol. 19, no. 4, pp. 407-413. https://doi.org/10.1007/s002990050748Rosell, G. In vitro mass tuberization as a contribution to potato micropropagation / G. Rosell, F. G. De Bertoldi, R. Tibia // Potato Research. – 1987. – Vol. 30, N 1. – P. 111–116. https://doi.org/ 10.1007/s10535-017-0715-xRosell G., De Bertoldi F. G., Tibia R. In vitro mass tuberization as a contribution to potato micropropagation. Potato Research, 1987, vol. 30 no. 1, pp. 111-116. https://doi.org/10.1007/s10535-017-0715-xИнновации в системе клонального микроразмножения картофеля / Б. В. Анисимов [и др.] // Картофель и овощи. – 2008. – № 4. – С. 26–27.Anisimov B. V., Smolegovets D. V., Smolegovets V. M., Gur’ev A. V. Innovations in the system of potato clonal micro-reproduction. Kartofel’ i ovoshchi = Potato and Vegetables, 2008, no. 4, pp. 26–27 (in Russian).Рекомендации по технологии выращивания in vitro микроклубней и их использования в процессе оригинального семеноводства / Всерос. науч.-исслед. ин-т картоф. хоз-ва ; сост.: Б. В. Анисимов, Д. В. Смолеговец, О. Н. Шатилова. – М. : ВНИИКХ, 2009. – 21 с.Anisimov B. V., Smolegovets D. V., Shatilova O. N. Recommendations for the technology of in vitro cultivation of microtubers and their use in the process of original seed production. Moscow, All-Russian Research Institute of Potato Production, 2009. 21 p. (in Russian).Овэс, Е. В. Выращивание in vitro микроклубней с применением контейнерной технологии / Е. В. Овэс, О. С. Колесова, Н. А. Фенина // Современная индустрия картофеля: состояние и перспективы развития : материалы VI межрегион. науч.-практ. конфер. / Всерос. науч.-исслед. ин-т картоф. хоз-ва ; редкол.: Б. В. Анисимов [и др.]. – Чебоксары, 2014. – С. 111–115.Oves E. V., Kolesova O. S., Fenina N. A. In vitro cultivation of microtubers using container technology. Sovremennaya industriya kartofelya: sostoyanie i perspektivy razvitiya: materialy VI mezhregional’noi nauchno-prakticheskoi konferentsii [Modern potato industry: state and development prospects: proceedings of the VI interregional scientific and practical conference]. Cheboksary, 2014, pp. 111–115 (in Russian).In vitro tuberization of potato cultivars as influenced by photoperiod, exogenous sucrose and cytokinin concentrations / E. F. Mahdi [et al.] // J. of the Saudi Soc. of Agr. Sciences. – 2004. – Vol. 17, N 1. – P. 25–35.Mahdi E. F., Hamad M., Al-Saad S., Sakina M. A. I. In vitro tuberization of potato cultivars as influenced by photoperiod, exogenous sucrose and cytokinin concentrations. Journal of the Saudi Society of Agricultural Sciences, 2004, vol. 17, no. 1, pp. 25-35.Kanwal, A. In vitro microtuberization of potato (Solanum tubersoum L.) cultivar Kurodaa new variety in Pakistan /A. Kanwal, A. Ali, K. Shoaib // Intern. J. of Agr. a. Biol. Engineering. – 2006. – Vol. 8, N 3. – P. 337–340.Kanwal A., Ali A., Shoaib K. In vitro microtuberization of potato (Solanum tubersoum L.) cultivar Kuroda – a new variety in Pakistan. International Journal of Agricultural and Biological Engineering, 2006, vol. 8, no. 3, pp. 337-340.Effect of chlorocholine chloride, sucrose and BAP on in vitro tuberization in potato (Solanum tuberosum L. cv. Cardinal) / I. Hussain [et al.] // Pakistan J. of Botany. – 2006. – Vol. 38, № 2. – P. 275–282.Hussain I., Chaudhry Z., Muhammad A., Asghar R., Naqvi S. M. S., Rashid H. Effect of chlorocholine chloride, sucrose and BAP on in vitro tuberization in potato (Solanum tuberosum L. cv.Cardinal). Pakistan Journal of Botany, 2006, vol. 38, no. 2, pp. 275-282.The effect of various concentrations of 6-benzylaminopurine (BAP) and sucrose on in vitro potato (Solanum tuberosum L.) microtuber induction / A. A. Imani [et al.] // Amer.-Eurasian J. of Agr. a. Environmental Sciences. – 2010. – Vol. 8, № 4. – P. 457–459.Imani A. A., Qhrmanzadeh R., Azimi J., Janpoor J. The effect of various concentrations of 6-benzylaminopurine (BAP) and sucrose on in vitro potato (Solanum tuberosum L.) microtuber induction. American-Eurasian Journal of Agricultural and Environmental Sciences, 2010, vol. 8, no. 4, pp. 457-459.Fufa, M. Microtuber induction of two potato (Solanum tuberosum L.) varieties / M. Fufa, M. Diro // Advances in Crop Science a. Technology. – 2014. – Vol. 2, N 2. – P. 1–4. https://doi.org/10.4172/2329-8863.1000122Fufa M., Diro M. Microtuber induction of two potato (Solanum tuberosum L.) varieties. Advances in Crop Science and Technology, 2014, vol. 2, no. 2, pp. 1-4. https://doi.org/10.4172/2329-8863.1000122Nhut, D. T. A novel in vitro hydroponic culture system for potato (Solanum tuberosum L.) microtuber production / D. T. Nhut, N.,H. Nguyen, D. T. T. Thuy // Scientia Horticulturae. – 2006. – Vol. 110, № 3. – P. 230–234. https://doi. org/10.1016/j.scienta.2006.07.027Nhut D. T., Nguyen N. H., Thuy D. T. T. A novel in vitro hydroponic culture system for potato (Solanum tuberosum L.) microtuber production. Scientia Horticulturae, 2006, vol. 110, no. 3, pp. 230-234. https://doi.org/10.1016/j.scienta.2006.07.027Efficient microtuber production of potato in modified nutrient spray bioreactor system / Md. Z. Rahman [et al.] // Scientia Horticulturae. – 2015. – Vol. 192. – P. 369–374. https://doi.org/10.1016/j.scienta.2015.06.014Rahman Md. Z., Shahinul S. M., Chowdhury A. N., Subramanian S. Efficient microtuber production of potato in modified nutrient spray bioreactor system. Scientia Horticulturae, 2015, vol. 192, pp. 369-374. https://doi.org/10.1016/j.scienta.2015.06.014Production of yam microtubers using a temporary immersion system / M. C. Jova [et al.] // Plant Cell, Tissue a. Organ Culture. – 2005. – Vol. 83, N 1. – P. 103–107. https://doi.org/10.1007/s11240-005-4853-zJova M. C., Pérez M. B., Pino A. S., Vega V. M., Torres J. L., Cabrera A. R., García M. G., DeVentura J. L. C., Kosky R. G. Production of yam microtubers using a temporary immersion system. Plant Cell, Tissue and Organ Culture, 2005, vol. 83, no. 1, pp. 103-107. https://doi.org/10.1007/s11240-005-4853-zEstrada, R. Induction of in vitro tubers in a broad range of potato genotypes / R. Estrada, P. Tovar, J. H. Dodds // Plant Cell, Tissue a. Organ Culture. – 1986. – Vol. 7, № 1. –P. 3–10. https://doi.org/10.1007/BF00043915Estrada R., Tovar P., Dodds J. H. Induction of in vitro tubers in a broad range of potato genotypes. Plant Cell, Tissue and Organ Culture, 1986, vol. 7, no. 1, pp. 3-10. https://doi.org/10.1007/BF00043915A simple method for mass production of potato microtubers using a bioreactor system / X. C. Piao [et al.] // Current Science. – 2003. – Vol. 84, N 8. – P. 1129–1132.Piao X. C., Chakrabarty D., Hahn E. J., Paek K. Y. A simple method for mass production of potato microtubers using a bioreactor system. Current Science, 2003, vol. 84, no. 8, pp. 1129-1132.Akita, M. Stimulation of potato (Solanum tuberosum L.) tuberization by semicontinous liquid medium surface level control / M. Akita, S. Takayama // Plant Cell Reports. – 1994. – Vol. 13, N 3–4. – P. 184–187. https://doi.org/10.1007/bf00239889Akita M., Takayama S. Stimulation of potato (Solanum tuberosum L.) tuberization by semicontinous liquid medium surface level control. Plant Cell Reports, 1994, vol. 13, no. 3-4, pp. 184-187. https://doi.org/10.1007/bf00239889Ebadi, M. Shoot micropropagation and microtuberization in potato (Solanum tuberosum L.) by the semi-continuous bioreactor / M. Ebadi, A. Iranbakhsh, G. B. Khaniki // Pakistan J. of Biol. Sciences. – 2007. – Vol. 10, N 6. – P. 861–867. https://doi.org/10.3923/pjbs.2007.861.867Ebadi M., Iranbakhsh A., Khaniki G. B. Shoot micropropagation and microtuberization in potato (Solanum tuberosum L.) by the semi-continuous bioreactor. Pakistan Journal of Biological Sciences, 2007, vol. 10, no. 6, pp. 861-867. https:// doi.org/10.3923/pjbs.2007.861.867In vitro tuberization of potato (Solanum tuberosum L.) variety Diacol Capiro in temporary immersion bioreactors and grow evaluation in field / N. M. Perez [et al.] // Ciencia. – 2008. – Vol. 16, N 3. – P. 288–295.Perez N. M., Restrepo D. C., Garcia J. D., Giraldo D. R. In vitro tuberization of potato (Solanum tuberosum L.) variety Diacol Capiro in temporary immersion bioreactors and grow evaluation in field. Ciencia, 2008, vol. 16, no. 3, pp. 288-295.Effects of illumination source, culture, ventilation, and sucrose on potato (Solanum tuberosum) microtuber production under short days / M. J. Alix [et al.] // Annals of Appl. Biology. – 2001. – Vol. 139, N 2. – P. 175–187. https://doi.org/10.1111/j.1744-7348.2001.tb00394.xAlix M. J., Savvides S., Blake J., Herrmann R., Hornung R. Effects of illumination source, culture, ventilation, and sucrose on potato (Solanum tuberosum) microtuber production under short days. Annals of Applied Biology, 2001, vol. 139, no. 2, pp. 175-187. https://doi.org/10.1111/j.1744-7348.2001.tb00394.xNovel bioreactor technology for mass propagation of potato microtubers / T. Kämäräinen-Karppinen [et al.] // Plant Cell, Tissue a. Organ Culture. – 2010. – Vol. 101, N 2. – P. 245–249. https://doi.org/10.1007/s11240-010-9679-7Kämäräinen-Karppinen T., Virtanen E., Rokka V.M., Pirttilä A. M. Novel bioreactor technology for mass propagation of potato microtubers. Plant Cell, Tissue and Organ Culture, 2010, vol. 101, no. 2, pp. 245-249. doi: 10.1007/s11240-010-9679-7Murashige, T. A revised medium for rapid growth and bio assays with tobacco tissue cultures / T. Murashige, F. Skoog // Physiologia Plantarum. – 1962. – Vol. 15, N 3. – P. 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.xMurashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 1962, vol. 15, no. 3, pp. 473-497. doi: 10.1111/j.1399-3054.1962.tb08052.xEwing, E. E. The role of hormones in potato (Solanum tuberosum L.) tuberization // Plant Hormones: physiology, biochemistry, and molecular biology / ed. P. J. Davies. – Dordrecht ; Boston, 1995. – P. 698–724. https://doi.org/10.1007/97894-011-0473-9_32Ewing E. E. The role of hormones in potato (Solanum tuberosum L.) tuberization. Plant Hormones: physiology, biochemistry, and molecular biology. Dordrecht, Boston, 1995, pp. 698-724. https://doi.org/10.1007/978-94-011-0473-9_32Palmer, C. E. Effect of kinetin on tuber formation on isolated stolons of Solanum tuberosum L. cultured in vitro / C. E. Palmer., O. E. Smith // Plant a. Cell Physiology. – 1970. – Vol. 11, № 2. – P. 303–314. https://doi.org/10.1093/oxfordjournals.pcp.a074511Palmer C. E., Smith O. E. Effect of kinetin on tuber formation on isolated stolons of Solanum tuberosum L. cultured in vitro. Plant and Cell Physiology, 1970, vol. 11, no. 2, pp. 303-314. https://doi.org/10.1093/oxfordjournals.pcp.a074511Performance of different protocols on in vitro tuberization in potato (Solanum tuberosum) / M. Zakaria [et al.] // Bangladesh J. of Agr. Research. – 2014. – Vol. 39, N 1. – P. 59–66. https://doi.org/10.3329/bjar.v39i1.20143Zakaria M., Hossain M. M., Khaleque Mian M. A., Hossain T. Performance of different protocols on in vitro tuberization in potato (Solanum tuberosum). Bangladesh Journal of Agricultural Research, 2014, vol. 39, no. 1, pp. 59-66. https:// doi.org/10.3329/bjar.v39i1.20143Effects of sucrose and growth regulators on the microtuberization of potato germplasm / S. Ali [et al.] // Pakistan J. of Botany. – 2018. – Vol. 50, N 2. – P. 763–768.Ali S., Khan N., Nouroz F., Erum S., Jatoi W. Effects of sucrose and growth regulators on the microtuberization of potato germplasm. Pakistan Journal of Botany, 2018, vol. 50, no. 2, pp. 763-768.Transformed potato plants as a model for studying the hormonal and carbohydrate regulation of tuberization / N. P. Aksenova [et al.] // Russ. J. of Plant Physiology. – 2000. – Vol. 47, N 3. – P. 370–379.Aksenova N. P., Konstantinova T. N., Golyanovskaya S. A., Kossmann J., Willmitzer L., Romanov G. A. Transformed potato plants as a model for studying the hormonal and carbohydrate regulation of tuberization. Russian Journal of Plant Physiology, 2000, vol. 47, no. 3, pp. 370-379.Effect of indole-3-acetic acid and kinetin on tuberization parameters of different cultivars and transgenic lines of potato in vitro / G. A. Romanov [et al.] // Plant Growth Regulation. – 2000. – Vol. 32, N 2–3. – P. 245–251. https://doi.org/10.1023/A:1010771510526Romanov G. A., Aksenova N. P., Konstantinova T. N., Golyanovskaya S. A., Kossmann J., Willmitzer L. Effect of indole-3-acetic acid and kinetin on tuberization parameters of different cultivars and transgenic lines of potato in vitro. Plant Growth Regulation, 2000, vol. 32, no. 2-3, pp. 245-251. https://doi.org/10.1023/A:1010771510526Dodds, J. H. Micropropagation of potato (Solanum tuberosum L.) / J. H. Dodds, D. Silva-Rodriguez, P. Tovar // Hightech and micropropagation III / ed. Y. P. S. Bajaj. – Berlin ; Heidelberg, 1992. – P. 91–106. – (Biotechnology in agriculture and forestry ; vol. 19). https://doi.org/10.1007/978-3-662-07770-2_6Dodds J. H., Silva-Rodriguez D., Tovar P. Micropropagation of potato (Solanum tuberosum L.). High-tech and micropropagation III. Biotechnology in agriculture and forestry, vol. 19. Berlin, Heidelberg, 1992, pp. 91-106. https://doi.org/10.1007/978-3-662-07770-2_6Khuri, S. Investigation into the role of sucrose in potato cv. Estima microtuber production in vitro / S. Khuri, J. Moorby // Annals of Botany. – 1995. – Vol. 75, N 3. – P. 295–303. https://doi.org/10.1006/anbo.1995.1024Khuri S., Moorby J. Investigation into the role of sucrose in potato cv. Estima microtuber production in vitro. Annals of Botany, 1995, vol. 75, no. 3, pp. 295-303. https://doi.org/10.1006/anbo.1995.1024Abbott, A. J. Potato tuber formation in vitro / A. J. Abbott, A. R. Belcher // Plant tissue culture and its agricultural applications / ed.: L. A. Withers, P. G. Alderson. – London, 1986. – P. 113–122.Abbott A. J., Belcher A. R. Potato tuber formation in vitro. Plant tissue culture and its agricultural applications. London, 1986, pp. 113–122.Garner N., The induction and development of potato microtubers in vitro on media free of growth regulating substances / N. Garner, J. Blake // Annals of Botany. – 1989. – Vol. 63, N 6. – P. 663–674. https://doi.org/10.1093/oxfordjournals.aob.a087795Garner N., Blake J. The induction and development of potato microtubers in vitro on media free of growth regulating substances. Annals of Botany, 1989, vol. 63, no. 6, pp. 663-674. https://doi.org/10.1093/oxfordjournals.aob.a087795Hussey, G. Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.) / G. Hussey, N. J. Stacey // Annals of Botany. – 1984. – Vol. 53, N 4. – P. 565–578. https://doi.org/10.1093/oxfordjournals.aob.a086720Hussey G., Stacey N. J. Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Annals of Botany, 1984, vol. 53, no. 4, pp. 565-578. https://doi.org/10.1093/oxfordjournals.aob.a086720Lawrence, C. H. A study of tuberization in the potato, Solanum tuberosum / C. H. Lawrence, W. G. Barker // Amer. Potato J. – 1963. – Vol. 40, N 10. – P. 349–356. https://doi.org/10.1007/bf02862742Lawrence C. H., Barker W. G. A study of tuberization in the potato, Solanum tuberosum. American Potato Journal, 1963, vol. 40, no. 10, pp. 349-356. https://doi.org/10.1007/bf02862742Effect of explant and sucrose on microtuber induction in potato cultivars / B. Fatima [et al.] // Intern. J. of Agriculture a. Biology. – 2005. – Vol. 7, N 1. – P. 63–66.Fatima B., Usman M., Ahmad I., Khan I. Effect of explant and sucrose on microtuber induction in potato cultivars. International Journal of Agriculture and Biology, 2005, vol. 7, no. 1, pp. 63-66.Role of sucrose, glucose and maltose on conventional potato micropropagation / M. H. Rahman [et al] // J. of Agr. Technology. – 2010. – Vol. 6, N 4. – P. 733–739.Rahman M. H., Islam R., Hossain M., Islam M. S. Role of sucrose, glucose and maltose on conventional potato micropropagation. Journal of Agricultural Technology, 2010, vol. 6, no. 4, pp. 733-739.Sarkar, D. Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro / D. Sarkar, S. Pandey, S. Sharma // Plant Cell, Tissue a. Organ Culture. – 2006. – Vol. 87, N 3. – P. 285–295. https://doi.org/10.1007/s11240-006-9166-3Sarkar D., Pandey S., Sharma S. Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell, Tissue and Organ Culture, 2006, vol. 87, no. 3, pp. 285-295. https://doi.org/10.1007/s11240-006-9166-3

The authors declare that there are no conflicts of interest present.