vestiagИзвестия Национальной академии наук Беларуси. Серия аграрных наукProceedings of the National Academy of Sciences of Belarus. Agrarian Series1817-72041817-7239The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1817-7204-2020-58-3-311-320vestiag-507Research ArticleЗЕМЛЯРОБСТВА І РАСЛІНАВОДСТВAAGRICULTURE AND PLANT CULTIVATIONАнализ полиморфизма генотипов земляники (Fragaria L.) по гену устойчивости к фитофторозной корневой гнили RPF1 для идентификации перспективных для селекции и садоводства формAnalysis of polymorphism of strawberry genotypes (Fragaria L.) according to the strawberry red root spot resistance gene RPF1 for identification of strawberry forms promising for breeding and horticulturehttps://orcid.org/0000-0001-9770-8731ЛыжинА. С.LyzhinA. S.

Лыжин Александр Сергеевич – кандидат сельскохозяйственных наук, ведущий научный сотрудник

ул. Мичурина, 30, 393774 Мичуринск, Тамбовская область

Alexander S. Lyzhin – Ph.D. (Agriculture)

30 Michurina Str., 393774 Michurinsk, Tambov region

Ranenburzhetc@yandex.ruЛукъянчукИ. В.Luk’yanchukI. V.

Лукъянчук Ирина Васильевна – кандидат сельскохозяйственных наук, старший научный сотрудник

ул. Мичурина, 30, 393774 Мичуринск, Тамбовская область

Irina V. Luk’yanchuk – Ph.D. (Agriculture)

30 Michurina Str., 393774 Michurinsk, Tambov region

irina.lk2011@yandex.ruФГБНУ «Федеральный научный центр имени И.В. Мичурина»I.V. Michurin, Federal Science Center202004082020583311320Copyright © Лыжин А.С., Лукъянчук И.В., 20202020Лыжин А.С., Лукъянчук И.В.Lyzhin A.S., Luk’yanchuk I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestiagr.belnauka.by/jour/article/view/507

Фитофторозная корневая гниль (Phytophthora fragariae var. fragariae Hickman) – одно из наиболее опасных заболеваний земляники в умеренном климатическом поясе. Важным этапом успешной селекционной работы по созданию устойчивых к фитофторозному увяданию сортов земляники является идентификация исходных форм, несущих гены устойчивости. Применение диагностических ДНК-маркеров целевых генов повысит надежность идентификации и эффективность селекционного процесса по созданию устойчивых генотипов земляники. Цель настоящего исследования – изучение полиморфизма дикорастущих видов р. Fragaria L. и сортов земляники садовой (F.çananassa Duch.) по гену Rpf1 устойчивости к фитофторозной корневой гнили с использованием молекулярных маркеров. Объектами исследования являлись дикорастущие виды F. orientalis Los., F. moschata Duch., F. ovalis Rydb., F. virginiana Duch. ssp. platypetala и сорта земляники садовой (F.çananassa Duch.) различного эколого-географического происхождения. Экстракция геномной ДНК земляники проводилась из молодых листьев согласно методу Puchooa. Для оценки аллельного состояния гена Rpf1 использовали маркеры SCAR-R1A (соответствует доминантному аллелю Rpf1) и OPO-16C (соответствует рецессивному аллелю rpf1). Маркер SCAR-R1A выявлен у дикорастущего вида F. virginiana Duch. ssp. platypetala (регион произрастания – Британская Колумбия, Канада), сорта земляники ананасной Былинная и перспективных отборных форм 62-41 (БылиннаяçФейерверк), 65-17, 65‑24 (Олимпийская надеждаçБылинная). Указанные генотипы характеризуются гетерозиготным генотипом по гену Rpf1 – Rpf1rpf1 (в генотипе присутствуют оба маркера) и могут использоваться в качестве источников устойчивости к фитофторозной корневой гнили для маркер-опосредованной селекции. У остальных изученных генотипов земляники маркер SCAR-R1A отсутствует, что предположительно свидетельствует об их рецессивном гомозиготном генотипе по гену Rpf1 (rpf1rpf1). Результаты исследований могут быть полезными селекционерам земляники садовой и исследователям биоразнообразия растений р. Fragaria.

Red root spot (Phytophthora fragariae var. fragariae Hickman) is one of the most important strawberry diseases in the temperate climate zone. Identification of forms, carrying resistance genes, is an important stage in breeding programs aimed at obtaining red root spot resistant strawberry varieties. Diagnostic DNA markers of target genes will increase reliability of identification and efficiency of strawberry breeding for the creation of resistant genotypes. The purpose of this study is analysis of polymorphism of wild species of Fragaria L. genus and strawberry varieties (F. × ananassa Duch.) according to the strawberry red root spot resistance gene Rpf1 using molecular markers. The research sunjects were the wild species F. orientalis Los., F. moschata Duch., F. ovalis Rydb., F. virginiana Duch. ssp. platypetala and strawberry varieties (F. × ananassa Duch.) of different ecological and geographic origin. Total genomic DNA of strawberry was extracted from the fresh leaves using the Puchooa method. To assess the allelic state of Rpf1 gene, SCAR-R1A marker (linked to the Rpf1 dominant allele) and OPO-16C marker (linked to the rpf1 recessive allele) have been used. SCAR-R1A marker was identified in wild species F. virginiana Duch. ssp. platypetala (vegetation region: British Columbia, Canada), pineapple strawberry varieties Bylinnaya and promising selected forms 62-41 (Bylinnaya × Feyyerverk), 65-17 and 65-24 (Olimpiyskaya nadezhda × Bylinnaya). These genotypes are characterized by heterozygous Rpf1rpf1 genotype according to Rpf1 gene (both markers are present in genotype) and can be used as red root spot resistance source in marker-assisted selection. In the remaining studied genotypes of strawberry, SCAR-R1A marker was not detected, which presumably indicated their homozygous recessive genotype rpf1rpf1 according to Rpf1 gene. The research results can be useful for breeders of strawberry and researchers of plant biodiversity of p. Fragaria.

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The authors declare that there are no conflicts of interest present.